Access the full text.
Sign up today, get DeepDyve free for 14 days.
M. Weiss, A. Kreusch, E. Schiltz, U. Nestel, W. Welte, J. Weckesser, G. Schulz (1991)
The structure of porin from Rhodobacter capsulatus at 1.8 Å resolutionFEBS Letters, 280
P. Spiegl, Clifford Feiner (1994)
Mycobacterium phlei Infection of the Foot: A Case ReportFoot & Ankle International, 15
Philip Draper (1998)
The outer parts of the mycobacterial envelope as permeability barriers.Frontiers in bioscience : a journal and virtual library, 3
E. Southern (1975)
Detection of specific sequences among DNA fragments separated by gel electrophoresis.Journal of molecular biology, 98 3
T. Lichtinger, B. Heym, E. Maier, H. Eichner, S. Cole, R. Benz (1999)
Evidence for a small anion‐selective channel in the cell wall of Mycobacterium bovis BCG besides a wide cation‐selective poreFEBS Letters, 454
B. Kartmann, Steffen Stengler, M. Niederweis (1999)
Porins in the Cell Wall ofMycobacterium tuberculosisJournal of Bacteriology, 181
K. Mdluli, R. Slayden, Yaping Zhu, S. Ramaswamy, Xi Pan, D. Mead, D. Crane, J. Musser, C. Barry (1998)
Inhibition of a Mycobacterium tuberculosis β-Ketoacyl ACP synthase by isoniazidScience, 280
A. Milner, W. Mack, K. Coates, J. Hill, I. Gill, P. Sheldrick (1990)
The sensitivity and specificity of a modified ELISA for the diagnosis of Johne's disease from a field trial in cattle.Veterinary microbiology, 25 2-3
B. Heym, W. Philipp, S. Cole (1994)
Mechanisms of drug resistance in Mycobacterium tuberculosis.Immunobiology, 191 4-5
Vincent JARLIERl, Hiroshi Nikaido (1990)
Permeability barrier to hydrophilic solutes in Mycobacterium cheloneiJournal of Bacteriology, 172
H. Tomioka (1998)
[Bacteriology of mycobacteria: taxonomic and morphological characteristics].Nihon rinsho. Japanese journal of clinical medicine, 56 12
F. Rieß, T. Lichtinger, R. Cseh, A. Yassin, K. Schaal, R. Benz (1998)
The cell wall porin of Nocardia farcinica: biochemical identification of the channel‐forming protein and biophysical characterization of the channel propertiesMolecular Microbiology, 29
D. Minnikin (1991)
Chemical principles in the organization of lipid components in the mycobacterial cell envelope.Research in microbiology, 142 4
Joaquim Trias, Vincent Jarlier, Roland Benz (1992)
Porins in the cell wall of mycobacteria.Science, 258 5087
V. Neuhoff, N. Arold, Dieter Taube, W. Ehrhardt (1988)
Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G‐250 and R‐250ELECTROPHORESIS, 9
S. Bech-Nielsen, J. Jørgensen, P. Ahrens, N. Feld (1992)
Diagnostic accuracy of a Mycobacterium phlei-absorbed serum enzyme-linked immunosorbent assay for diagnosis of bovine paratuberculosis in dairy cowsJournal of Clinical Microbiology, 30
S. Bech-Nielsen, W. Shulaw, P. Frandsen, J. Jørgensen, N. Feld, P. Ahrens (1992)
Comparison of subjective and objective test evaluations for use of Mycobacterium phlei-adsorbed serum in a dot-enzyme-linked immunosorbent assay for diagnosis of paratuberculosis in cattle.American journal of veterinary research, 53 8
F. Rieß, R. Benz (2000)
Discovery of a novel channel-forming protein in the cell wall of the non-pathogenic Nocardia corynebacteroides.Biochimica et biophysica acta, 1509 1-2
S. Cole, R. Brosch, J. Parkhill, T. Garnier, C. Churcher, D. Harris, S. Gordon, K. Eiglmeier, S. Gas, C. Barry, F. Tekaia, K. Badcock, D. Basham, David Brown, T. Chillingworth, R. Connor, R. Davies, K. Devlin, T. Feltwell, S. Gentles, N. Hamlin, S. Holroyd, T. Hornsby, K. Jagels, A. Krogh, J. Mclean, S. Moule, L. Murphy, K. Oliver, J. Osborne, M. Quail, M. Rajandream, J. Rogers, S. Rutter, K. Seeger, J. Skelton, R. Squares, S. Squares, J. Sulston, K. Taylor, S. Whitehead, B. Barrell (1998)
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequenceNature, 393
I. Yano, K. Saito (1972)
Gas chromatographic and mass spectrometric analysis of molecular species of corynomycolic acids from Corynebacterium ulceransFEBS Letters, 23
J. Trias, R. Benz (1994)
Permeability of the cell wall of Mycobacterium smegmatisMolecular Microbiology, 14
T. Lichtinger, Gilana Reiss, R. Benz (2000)
Biochemical Identification and Biophysical Characterization of a Channel-Forming Protein fromRhodococcus erythropolisJournal of Bacteriology, 182
R. Benz (1988)
Structure and function of porins from gram-negative bacteria.Annual review of microbiology, 42
R. Benz, K. Janko, W. Boos, P. Läuger (1978)
Formation of large, ion-permeable membrane channels by the matrix protein (porin) of Escherichia coli.Biochimica et biophysica acta, 511 3
J. Liu, C. Barry, G. Besra, H. Nikaido (1996)
Mycolic Acid Structure Determines the Fluidity of the Mycobacterial Cell Wall*The Journal of Biological Chemistry, 271
R. Benz, K. Janko, P. Läuger (1979)
Ionic selectivity of pores formed by the matrix protein (porin) of Escherichia coli.Biochimica et biophysica acta, 551 2
C. Barry, K. Mdluli (1996)
Drug sensitivity and environmental adaptation of mycobacterial cell wall components.Trends in microbiology, 4 7
M. Daffé, P. Brennan, M. McNeil (1990)
Predominant structural features of the cell wall arabinogalactan of Mycobacterium tuberculosis as revealed through characterization of oligoglycosyl alditol fragments by gas chromatography/mass spectrometry and by 1H and 13C NMR analyses.The Journal of biological chemistry, 265 12
A. Yassin, F. Rainey, J. Burghardt, H. Brzezinka, S. Schmitt, P. Seifert, O. Zimmermann, H. Mauch, D. Gierth, I. Lux, K. Schaal (1997)
Tsukamurella tyrosinosolvens sp. nov.International journal of systematic bacteriology, 47 3
J. Aguilar, E. Sánchez, C. Carrillo, G. Alarcón, A. Silicani (1989)
Septic arthritis due to Mycobacterium phlei presenting as infantile Reiter's syndrome.The Journal of rheumatology, 16 10
H. Chambers, D. Moreau, D. Yajko, C. Miick, Cindy Wagner, C. Hackbarth, S. Kocagöz, E. Rosenberg, W. Hadley, H. Nikaido (1995)
Can penicillins and other beta-lactam antibiotics be used to treat tuberculosis?Antimicrobial Agents and Chemotherapy, 39
H. Schindler, J. Rosenbusch (1981)
Matrix protein in planar membranes: clusters of channels in a native environment and their functional reassembly.Proceedings of the National Academy of Sciences of the United States of America, 78 4
D. Stahl, J. Urbance (1990)
The division between fast- and slow-growing species corresponds to natural relationships among the mycobacteriaJournal of Bacteriology, 172
Kozo Ochi (1995)
Phylogenetic analysis of mycolic acid-containing wall-chemotype IV actinomycetes and allied taxa by partial sequencing of ribosomal protein AT-L30.International journal of systematic bacteriology, 45 4
E. Stackebrandt, F. Rainey, N. Ward-Rainey (1997)
Proposal for a New Hierarchic Classification System, Actinobacteria classis nov.International Journal of Systematic and Evolutionary Microbiology, 47
D. van, Soolingen, P. Hermans, DE 'PETRAE.W., Haas, D. Soll, J. EMBDENl (1991)
Occurrence and stability of insertion sequences in Mycobacterium tuberculosis complex strains: evaluation of an insertion sequence-dependent DNA polymorphism as a tool in the epidemiology of tuberculosisJournal of Clinical Microbiology, 29
C. Raynaud, M. Lanéelle, Ryan Senaratne, P. Draper, G. Lanéelle, M. Daffé (1999)
Mechanisms of pyrazinamide resistance in mycobacteria: importance of lack of uptake in addition to lack of pyrazinamidase activity.Microbiology, 145 ( Pt 6)
P. Brennan, H. Nikaido (1995)
The envelope of mycobacteria.Annual review of biochemistry, 64
D. Minnikin, P. Patel (1974)
Mycolic acids of representative strains of Nocardia and the ‘rhodochrous’ complexFEBS Letters, 39
MC Filion, P. Lépicier, A. Morales, NC Phillips (1999)
Mycobacterium phlei cell wall complex directly induces apoptosis in human bladder cancer cellsBritish Journal of Cancer, 79
Joaquim Trias, Roland Benz (1993)
Characterization of the channel formed by the mycobacterial porin in lipid bilayer membranes. Demonstration of voltage gating and of negative point charges at the channel mouth.The Journal of biological chemistry, 268 9
H. Nikaido, E. Rosenberg (1981)
Effect on solute size on diffusion rates through the transmembrane pores of the outer membrane of Escherichia coliThe Journal of General Physiology, 77
G. Gray, E. Ribi, D. Granger, R. Parker, I. Azuma, K. Yamamoto (1975)
Immunotherapy of cancer: tumor suppression and regression by cell walls of Mycobacterium phlei attached to oil droplets.Journal of the National Cancer Institute, 55 3
A. Nelson, D. Mcquarrie (1975)
The effect of discrete charges on the electrical properties of membranes. II.Journal of theoretical biology, 93 3
A. Roth, M. Fischer, M. Hamid, Sabine Michalke, W. Ludwig, H. Mauch (1998)
Differentiation of Phylogenetically Related Slowly Growing Mycobacteria Based on 16S-23S rRNA Gene Internal Transcribed Spacer SequencesJournal of Clinical Microbiology, 36
M. Niederweis, S. Ehrt, Christian Heinz, Uta Klöcker, S. Karosi, K. Swiderek, L. Riley, R. Benz (1999)
Cloning of the mspA gene encoding a porin from Mycobacterium smegmatisMolecular Microbiology, 33
Jun Liu, E. Rosenberg, Hiroshi Nikaido (1995)
Fluidity of the lipid domain of cell wall from Mycobacterium chelonae.Proceedings of the National Academy of Sciences of the United States of America, 92 24
Eugene Renkin (1954)
FILTRATION, DIFFUSION, AND MOLECULAR SIEVING THROUGH POROUS CELLULOSE MEMBRANESThe Journal of General Physiology, 39
T. Lichtinger, A. Burkovski, Michael Niederweis, Reinhard Krämer, Roland Benz (1998)
Biochemical and biophysical characterization of the cell wall porin of Corynebacterium glutamicum: the channel is formed by a low molecular mass polypeptide.Biochemistry, 37 43
G. Menestrina, R. Antolini (1981)
Ion transport through hemocyanin channels in oxidized cholesterol artificial bilayer membranes.Biochimica et biophysica acta, 643 3
U. Laemmli (1970)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 227
S. Cole, K. Eiglmeier, J. Parkhill, K. James, N. Thomson, P. Wheeler, N. Honoré, T. Garnier, C. Churcher, D. Harris, K. Mungall, D. Basham, D. Brown, T. Chillingworth, R. Connor, R. Davies, K. Devlin, S. Duthoy, T. Feltwell, A. Fraser, N. Hamlin, S. Holroyd, T. Hornsby, K. Jagels, C. Lacroix, J. Maclean, S. Moule, L. Murphy, K. Oliver, M. Quail, M. Rajandream, Kim Rutherford, S. Rutter, K. Seeger, S. Simon, M. Simmonds, J. Skelton, R. Squares, S. Squares, K. Stevens, K. Taylor, S. Whitehead, J. Woodward, B. Barrell (2001)
Massive gene decay in the leprosy bacillusNature, 409
The gram-positive bacterium Mycobacterium phlei was treated with detergents. Reconstitution experiments using lipid bilayers suggested that the detergent extracts contain a channel forming protein. The protein was purified to homogeneity by preparative SDS-PAGE and identified as a protein with an apparent molecular mass of about 135 kDa. The channel-forming unit dissociated into subunits with a molecular mass of about 22 kDa when it was boiled in 80% dimethylsulfoxid (DMSO). The channel has on average a single channel conductance of 4.5 nS in 1 m KCl and is highly voltage-dependent in an asymmetric fashion when the protein is added to only one side of the membrane. Zero-current membrane potential measurements with different salts implied that the channel is highly cation-selective because of negative point charges in or near the channel mouth. Analysis of the single-channel conductance as a function of the hydrated cation radii using the Renkin correction factor and the effect of the negative point charges on the single-channel conductance suggest that the diameter of the cell wall channel is about 1.8 to 2.0 nm. The channel properties were compared with those of other members of the mycolata and suggest that these channels share common features. Southern blots demonstrated that the chromosome of M. phlei and other mycolata tested contain homologous sequences to mspA (gene of the cell wall porin of Mycobacterium smegmatis).
The Journal of Membrane Biology – Springer Journals
Published: Jul 15, 2001
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.