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Monatsh Chem (2017) 148:1605–1611 DOI 10.1007/s00706-017-1965-1 ORIGINAL PAPER Study of polyaniline-coated silica gel as a stationary phase in different modes of capillary liquid chromatography 1 1 Luka ´ s ˇ Taraba Toma ´ s ˇ Kr ˇ´ ız ˇek Received: 3 January 2017 / Accepted: 19 March 2017 / Published online: 3 July 2017 Springer-Verlag Wien 2017 Abstract Separation potential of a stationary phase based Graphical abstract on polyaniline-coated silica gel porous spherical particles was investigated in different chromatographic modes of capillary liquid chromatography. L-Tryptophan and three of its structural derivatives with modified amino or carboxylic functionality were used to investigate the retention factor curves under different mobile phase composition including pH variation. Some retention curves pass minimum which indicates that more than one chromatographic mode can be employed to retain the relevant solute. The positional iso- Keywords High pressure liquid chromatography mers of aminoacetophenone and caffeine and its Material science Stationary phase demethylated analogs—theobromine and theophylline— Structure–activity relationships Polyaniline were used as separation probes in normal phase, hydro- philic interaction liquid chromatography, and reversed phase modes. The stationary phase exhibits the mixed- Introduction mode retention mechanism; therefore, it is not easy to predict the elution order
Monatshefte für Chemie - Chemical Monthly – Springer Journals
Published: Jul 3, 2017
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