ISSN 10227954, Russian Journal of Genetics, 2011, Vol. 47, No. 7, pp. 770–774. © Pleiades Publishing, Inc., 2011.
The existence of experimental evidence about diver
sity of chemical substances that are of natural origin and
their capability to achieve (realize) different biological
effects, because they can serve as a raw material in phar
maceutical industry, are the reasons for more studious
approach in research of consequences of herbal metabo
lit interaction with hereditary material.
plant is a small genus of the
family Anacardiaceae with two species:
Raf., American smoketree. Smoketree comes from
southern Europe, central China and Himalayas.
Plants in the family Anacardiaceae have a long history
of use by peoples for medicinal and other uses.
it tradicionally belived to be usefil as an anti
microbial treatment, used in the form of external
washes . In the folk medicine
used as antiseptic, antiinflamatory, antimicrobial,
antihaemorragic effects, woundhealing , against
diarrrhoea, paradontosis, gastric and duodenal ulcer
. Extract of
is effective for
enhancing the elasticity of the skin and/or treating
wounds, including the inhibition of the appearance of
scars . A yellow/orange dye can be obtained from
the root and stem and can be used for fabric dying. The
leaves and bark are a good source of tannins , which
can have influence on DNA function .
Chemical analyses of the plant
which is often used in traditional medicine, show that
is rich with polyphenols, flavonoids and tannins [7, 8]
The article is published in the original.
and it is established that they can influence on bio
chemical and physiological functions of living systems
that are on different leveals of complexity [9, 10]. Sci
entific data confirm that this metabolits have antioxi
dative, antiphugal and antiinflamatory capability [11,
12], but there is little information about genotoxicity
and/or antigenotoxicity of the extract of this plant .
Test for sex linked lethals (sexlinked recessive
lethal test—SLRL) is shorttermed, highly sensitive
and is used for detection of mutations on X chromo
some in germinative cell lines of premeiotic (sperma
tocites) and postmeiotic degress (spermatides and
spermatozoides) in the conditions of
high correlation between carcinogenic and mutagenic
activity in SLRL test is the reason of its frequent using
in genotoxicity research. Hereditary changes are mea
sured on nontreated offsprins (posterity) so that we
get a real hereditary effect, and microsome enzyme
reactions of the used model system, enable results
extrapolation that is danger estimation on man .
We have used SLRL test on
genotoxicity and antigeno
extract, prepared in two
concentrations. For positive control and posttreat
ment we used 0.75 ppm of EMS which is a proven
mutagene from alkilating agents class .
In this study we show results of investigations of geno
toxic and antigenotoxic effects of
anol extract on mutagenicity induced with EMS on X
Study of Genotoxicity and Antigenotoxicity of the
Methanol Extract by
SexLinked Recessive Lethal Test
, S. Matic
, G. Ðelic
, M. Mihailovic
, D. Bogojevic
, and S. Solujic
Department of Biology and Ecology, Faculty of Science, University of Kragujevac, 34000, Serbia
Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Serbia
Department of Chemistry, Faculty of Science, University of Kragujevac, Serbia
Received October 29, 2010
—The genotoxic and antigenotoxic effects of
Scop. methanol extract was investi
gated using the
sexlinked recessive lethal (or SLRL) test. The results presented here show that the
methanol extract of
in a concentration of 5% and artificial chemical agent ethyl methane
sulfonate EMS (0.75 ppm) induce recessive lethal mutations on Xchromosome on
in all broods (I, II and III). Posttreatment with lower concentration of the methanol extract of
(2%) was effective in reducing genotoxicity of mutagen.