Study and Understanding Behavior of Alginate-Inulin Synbiotics Beads for Protection and Delivery of Antimicrobial-Producing Probiotics in Colonic Simulated Conditions

Study and Understanding Behavior of Alginate-Inulin Synbiotics Beads for Protection and Delivery... According to the World Health Organization (WHO), using antibiotics as growth promoters for livestock—particularly swine—is the principal cause of antibiotic resistance. It is therefore clear that finding an alternative to antibiotics becomes an emergency. Hundreds of recent studies have appointed probiotics as potential candidates to replace or to be used in combination with antibiotics. However, bringing probiotics alive to the colon—their site of action—remains a big challenge because of different physiological barriers encountered in proximal gastrointestinal tract (GIT) such as acidic pH and bile salts that may affect the viability of probiotic cultures. To overcome this problem, in previous studies, we developed and characterize a synbiotic formula consisting of beads of a mixture of alginate and inulin. Three potential probiotics strains namely Pediococcus acidilactici UL5 (UL5), Lactobacillus reuteri (LR), and Lactobacillus salivarius (LS) were encapsulated to study their release and the behavior of this synbiotic formula throughout the GIT using in vitro models. The survival and the release of bacteria from beads were studied by specific PMA-qPCR counting. The microscopic aspects of the beads were studied using scanning electron microscopy (SEM). Moreover, the microbial dynamics inside beads were studied by fluorescence microscopy using the live/dead test. Our results have shown that the beads containing 5% inulin were the most stable in the stomach and throughout the small intestine. However, beads were completely degraded in approximately 3 h of incubation in the fermented medium that mimic the colon. These results were confirmed by SEM and fluorescence microscopy images. Therefore, it can be stated that the AI5 formulation well protected the bacteria in the upper part of the digestive tract and allowed their controlled release in the colon. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Probiotics and Antimicrobial Proteins Springer Journals

Study and Understanding Behavior of Alginate-Inulin Synbiotics Beads for Protection and Delivery of Antimicrobial-Producing Probiotics in Colonic Simulated Conditions

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Publisher
Springer Journals
Copyright
Copyright © 2017 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Chemistry; Chemistry/Food Science, general; Applied Microbiology; Microbiology; Protein Science; Nutrition
ISSN
1867-1306
eISSN
1867-1314
DOI
10.1007/s12602-017-9355-x
Publisher site
See Article on Publisher Site

Abstract

According to the World Health Organization (WHO), using antibiotics as growth promoters for livestock—particularly swine—is the principal cause of antibiotic resistance. It is therefore clear that finding an alternative to antibiotics becomes an emergency. Hundreds of recent studies have appointed probiotics as potential candidates to replace or to be used in combination with antibiotics. However, bringing probiotics alive to the colon—their site of action—remains a big challenge because of different physiological barriers encountered in proximal gastrointestinal tract (GIT) such as acidic pH and bile salts that may affect the viability of probiotic cultures. To overcome this problem, in previous studies, we developed and characterize a synbiotic formula consisting of beads of a mixture of alginate and inulin. Three potential probiotics strains namely Pediococcus acidilactici UL5 (UL5), Lactobacillus reuteri (LR), and Lactobacillus salivarius (LS) were encapsulated to study their release and the behavior of this synbiotic formula throughout the GIT using in vitro models. The survival and the release of bacteria from beads were studied by specific PMA-qPCR counting. The microscopic aspects of the beads were studied using scanning electron microscopy (SEM). Moreover, the microbial dynamics inside beads were studied by fluorescence microscopy using the live/dead test. Our results have shown that the beads containing 5% inulin were the most stable in the stomach and throughout the small intestine. However, beads were completely degraded in approximately 3 h of incubation in the fermented medium that mimic the colon. These results were confirmed by SEM and fluorescence microscopy images. Therefore, it can be stated that the AI5 formulation well protected the bacteria in the upper part of the digestive tract and allowed their controlled release in the colon.

Journal

Probiotics and Antimicrobial Proteins – Springer Journals

Published: Nov 27, 2017

References

  • Pharmaceutical Science and Technology, Garg, Sanj
    Perumal, OP; Haywood, A; Glass, B; Ho, PC-L
  • Biopharmaceutics and pharmacokinetics in drug research
    Panchagnula, R; Thomas, NS
  • Two replicate randomized, double-blind, placebo-controlled trials of the time to onset of pain relief in the acute treatment of migraine with a fast-disintegrating/rapid-release formulation of sumatriptan tablets
    Sheftell, FD; Dahlöf, CGH; Brandes, JL; Agosti, R; Jones, MW; Barrett, PS
  • Overall mechanism behind matrix sustained release (SR) tablets prepared with hydroxypropyl methylcellulose 2910
    Tahara, K; Yamamoto, K; Nishihata, T
  • Colon targeted drug delivery systems: a review on primary and novel approaches
    Philip, AK; Philip, B
  • Mechanisms of drug release from cyclodextrin complexes
    Stella, VJ; Rao, VM; Zannou, EA; Zia, V
  • Review article: dual delayed release formulation of dexlansoprazole MR, a novel approach to overcome the limitations of conventional single release proton pump inhibitor therapy
    Metz, DC; Vakily, M; Dixit, T; Mulford, D
  • Pourquoi la flore intestinale a-t-elle vocation à devenir médicament ?
    BOURLIOUX, P; Corthier, G; Gobert, J-G; Butel, M-J
  • Intégrer la caractérisation du microbiote digestif dans le phénotypage de l ’ animal de rente : vers un nouvel outil de maîtrise de la santé en élevage ?
    Calenge, F; Martin, C; Floch, NLE; Phocas, F; Morgavi, D; Sup, V; Ouest, A
  • Changes in caecal microbiota and mucosal morphology of weaned pigs
    Castillo, M; Martín-Orúe, SM; Nofrarías, M; Manzanilla, EG; Gasa, J
  • Permeabilized probiotic lactobacillus plantarum as a source of β-galactosidase for the synthesis of prebiotic galactooligosaccharides
    Gobinath, D; Prapulla, SG
  • Microencapsulation of probiotics for gastrointestinal delivery
    Cook, MT; Tzortzis, G; Charalampopoulos, D; Khutoryanskiy, VV
  • Macrobeads based-polysaccharides: development and morphological analysis
    Gbassi, GK; Atheba, P; Yolou, FS; Vandamme, T
  • Co-encapsulation of probiotics with prebiotics on alginate matrix and its effect on viability in simulated gastric environment
    Sathyabama, S; Ranjith Kumar, M; Bruntha Devi, P; Vijayabharathi, R; Brindha Priyadharisini, V
  • Probiotic viability, physicochemical characteristics and acceptability during refrigerated storage of clarified apple juice supplemented with lactobacillus paracasei ssp. paracasei and oligofructose in different package type
    Pimentel, TC; Madrona, GS; Garcia, S; Prudencio, SH
  • Physicochemical and microbiological properties of fermented lamb sausages using probiotic lactobacillus plantarum IIA-2C12 as starter culture
    Arief, II; Wulandari, Z; Aditia, EL; Baihaqi, M
  • Microbiological and physicochemical quality of fresh-cut apple enriched with the probiotic strain lactobacillus rhamnosus GG
    Alegre, I; Viñas, I; Usall, J; Anguera, M; Abadias, M
  • In vitro gastrointestinal model (TIM) with predictive power, even for infants and children?
    Havenaar, R; Anneveld, B; Hanff, LM; Wildt, SN; Koning, BAE; Mooij, MG; Lelieveld, JPA; Minekus, M
  • Dynamic gastro-intestinal in vitro model (TIM) for reliable prediction of stability, availability for absorption, and luminal efficacy of clinical foods and ingredients
    Havenaar, R; Bellmann, S; Zeijdner, E
  • The microbiome explored: recent insights and future challenges
    Blaser, M; Bork, P; Fraser, C; Knight, R; Wang, J
  • Reviving the superorganism
    Wilson, DS; Sober, E
  • Genome sequence of the vertebrate gut symbiont lactobacillus reuteri ATCC 53608
    Heavens, D; Tailford, LE; Crossman, L; Jeffers, F; MacKenzie, DA; Caccamo, M; Juge, N
  • Simple method of purification and sequencing of a bacteriocin produced by Pediococcus acidilactici UL5
    Daba, H; Lacroix, C; Huang, J; Simard, RE; Lemieux, L
  • The influence of selected parameters on the size and shape of alginate beads prepared by ionotropic gelation
    Smrdel, P; Bogataj, M; Mrhar, A
  • In vitro dissolution testing with flow-through method: a technical note
    Gao, Z
  • Validation of a three-stage compound continuous culture system for investigating the effect of retention time on the ecology and metabolism of bacteria in the human colon
    Macfarlane, GT; Macfarlane, S; Gibson, GR
  • In vitro continuous fermentation model (PolyFermS) of the swine proximal colon for simultaneous testing on the same gut microbiota
    Tanner, SA; Zihler Berner, A; Rigozzi, E; Grattepanche, F; Chassard, C; Lacroix, C
  • On Lactococcus lactis UL719 competitivity and nisin (Nisaplin(®)) capacity to inhibit Clostridium Difficile in a model of human colon
    Lay, C; Fernandez, B; Hammami, R; Ouellette, M; Fliss, I
  • Phenotypic stability of bovine articular chondrocytes after long-term culture in alginate beads
    Häuselmann, HJ; Fernandes, RJ; Mok, SS; Schmid, TM; Block; Aydelotte, MB; Kuettner, KE; Thonar, EJ
  • Rapid quantification of viable campylobacter bacteria on chicken carcasses, using real-time PCR and propidium monoazide treatment, as a tool for quantitative risk assessment
    Josefsen, MH; Löfström, C; Hansen, TB; Christensen, LS; Olsen, JE; Hoorfar, J
  • Survival and metabolic activity of Pediocin producer Pediococcus acidilactici UL5: its impact on intestinal microbiota and listeria monocytogenes in a model of the human terminal ileum
    Fernandez, B; Savard, P; Fliss, I
  • Identification of Pediococcus acidilactici and Pediococcus pentosaceus based on 16S rRNA and ldhD gene-targeted multiplex PCR analysis
    Mora, D; Fortina, MG; Parini, C; Manachini, PL
  • Real-time quantitative PCR measurement of ileal lactobacillus salivarius populations from broiler chickens to determine the influence of farming practices
    Harrow, SA; Ravindran, V; Butler, RC; Marshall, JW; Tannock, GW
  • Obesity-associated gut microbiota is enriched in lactobacillus reuteri and depleted in Bifidobacterium animalis and Methanobrevibacter smithii
    Million, M; Maraninchi, M; Henry, M; Armougom, F; Richet, H; Carrieri, P; Valero, R; Raccah, D; Vialettes, B; Raoult, D
  • Vivo study on the effectiveness of pediocin PA-1 and Pediococcus acidilactici UL5 at inhibiting listeria monocytogenes
    Dabour, N; Zihler, A; Kheadr, E; Lacroix, C; Fliss, I
  • Pediococcus acidilactici UL5 and Lactococcus lactis ATCC 11454 are able to survive and express their bacteriocin genes under simulated gastrointestinal conditions
    Fernandez, B; Hammami, R; Savard, P; Jean, J; Fliss, I
  • Survival and persistence of host-associated Bacteroidales cells and DNA in comparison with Escherichia Coli and enterococcus in freshwater sediments as quantified by PMA-qPCR and qPCR
    Kim, M; Wuertz, S
  • Optimization and validation of a PMA qPCR method for Escherichia Coli quantification in primary production
    Truchado, P; Gil, MI; Kostic, T; Allende, A
  • Qualification and validation of USP apparatus 4
    Kauffman, JS
  • Dissolution study of active pharmaceutical ingredients using the flow through apparatus USP 4
    Beyssac, E; Lavigne, J
  • United States pharmacopeia safety evaluation of spirulina
    Marles, RJ; Barrett, ML; Barnes, J; Chavez, ML; Gardiner, P; Ko, R; Mahady, GB; Low Dog, T; Sarma, ND; Giancaspro, GI; Sharaf, M; Griffiths, J
  • Rapid dissolution of propofol emulsions under sink conditions
    Damitz, R; Chauhan, A
  • Examination of drug release and distribution from drug-eluting stents with a vessel-simulating flow-through cell
    Seidlitz, A; Nagel, S; Semmling, B; Grabow, N; Martin, H; Senz, V; Harder, C; Sternberg, K; Schmitz, K-P; Kroemer, HK; Weitschies, W
  • Micelle dynamic simulation and physicochemical characterization of biorelevant media to reflect gastrointestinal environment in fasted and fed states
    Xie, X; Cardot, J-M; Garrait, G; Thery, V; El-Hajji, M; Beyssac, E
  • A standardised static in vitro digestion method suitable for food - an international consensus
    Minekus, M; Alminger, M; Alvito, P; Ballance, S; Bohn, T; Bourlieu, C; Carrière, F; Boutrou, R; Corredig, M; Dupont, D; Dufour, C; Egger, L; Golding, M; Karakaya, S; Kirkhus, B; Feunteun, S; Lesmes, U; Macierzanka, A; Mackie, A; Marze, S; McClements, DJ; Ménard, O; Recio, I; Santos, CN; Singh, RP; Vegarud, GE; Wickham, MSJ; Weitschies, W; Brodkorb, A
  • Survival, growth and toxicity of Microcystis Aeruginosa PCC 7806 in experimental conditions mimicking some features of the human gastro-intestinal environment
    Stefanelli, M; Vichi, S; Stipa, G; Funari, E; Testai, E; Scardala, S; Manganelli, M
  • Enzyme/pH dual sensitive polymeric nanoparticles for targeted drug delivery to the inflamed colon
    Naeem, M; Kim, W; Cao, J; Jung, Y; Yoo, J-W
  • Simulated gastrointestinal digestion and in vitro colonic fermentation of spent coffee (Coffea Arabica L.): bioaccessibility and intestinal permeability
    Campos-Vega, R; Vázquez-Sánchez, K; López-Barrera, D; Loarca-Piña, G; Mendoza-Díaz, S; Oomah, BD

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