Access the full text.
Sign up today, get DeepDyve free for 14 days.
I. Papp, V. Iglesias, E. Moscone, S. Michalowski, S. Spiker, Y. Park, M. Matzke, A. Matzke (1996)
Structural instability of a transgene locus in tobacco is associated with aneuploidy.The Plant journal : for cell and molecular biology, 10 3
S. Buck, C. Wilde, M. Montagu, A. Depicker (2000)
T-DNA vector backbone sequences are frequently integrated into the genome of transgenic plants obtained by Agrobacterium-mediated transformationMolecular Breeding, 6
H. Vaucheret, M. Fagard (2001)
Transcriptional gene silencing in plants: targets, inducers and regulators.Trends in genetics : TIG, 17 1
Bénédicte Charrier, C. Scollan, S. Ross, E. Zubko, P. Meyer (2000)
Co-silencing of homologous transgenes in tobaccoMolecular Breeding, 6
Heidi Scrable, Peter Stambrook (1999)
A genetic program for deletion of foreign DNA from the mammalian genome.Mutation research, 429 2
M. Matzke, M. Mette, J. Jakowitsch, T. Kanno, E. Moscone, J. van der Winden, A. Matzke (2001)
Transvection-like effects in plants: evidence that DNA pairing leads to trans-activation of silencing of complex heteroalleles in tobaccoGenetics, 158
A. Wolters, R. Visser (2000)
Gene silencing in potato: allelic differences and effect of ploidyPlant Molecular Biology, 43
S. Henikoff, Luca Comai (1998)
A DNA methyltransferase homolog with a chromodomain exists in multiple polymorphic forms in Arabidopsis.Genetics, 149 1
S. Jacobsen, H. Sakai, E. Finnegan, Xiaofeng Cao, E. Meyerowitz (2000)
Ectopic hypermethylation of flower-specific genes in ArabidopsisCurrent Biology, 10
J. Sambrook, E. Fritsch, T. Maniatis (2001)
Molecular Cloning: A Laboratory Manual
Y. Yoshioka, S. Matsumoto, S. Kojima, K. Ohshima, N. Okada, Y. Machida (1993)
Molecular characterization of a short interspersed repetitive element from tobacco that exhibits sequence homology to specific tRNAs.Proceedings of the National Academy of Sciences of the United States of America, 90 14
M. Mette, W. Aufsatz, J. Winden, M. Matzke, A. Matzke (2000)
Transcriptional silencing and promoter methylation triggered by double‐stranded RNAThe EMBO Journal, 19
Steven Jacobsen, E. Meyerowitz (1997)
Hypermethylated SUPERMAN epigenetic alleles in arabidopsis.Science, 277 5329
D. Selinger, V. Chandler (2001)
B-Bolivia, an allele of the maize b1 gene with variable expression, contains a high copy retrotransposon-related sequence immediately upstream.Plant physiology, 125 3
A. Matzke, M. Matzke (1986)
A set of novel Ti plasmid-derived vectors for the production of transgenic plantsPlant Molecular Biology, 7
P. Waterhouse, Ming-Bo Wang, T. Lough (2001)
Gene silencing as an adaptive defence against virusesNature, 411
V. Kuhrová, M. Bezděk, B. Vyskot, B. Koukalová, J. Fajkus (1991)
Isolation and characterization of two middle repetitive DNA sequences of nuclear tobacco genomeTheoretical and Applied Genetics, 81
A. Kovařík, B. Koukalová, K. Lim, R. Matyášek, Conrad Lichtenstein, A. Leitch, Milan Bezděk (2004)
Comparative analysis of DNA methylation in tobacco heterochromatic sequencesChromosome Research, 8
Susan Michalowski, George Allen, G. Hall, William Thompson, S. Spiker (1999)
Characterization of randomly-obtained matrix attachment regions (MARs) from higher plants.Biochemistry, 38 39
M. Mette, J. Winden, M. Matzke, A. Matzke (1999)
Production of aberrant promoter transcripts contributes to methylation and silencing of unlinked homologous promoters in transThe EMBO Journal, 18
W. Aufsatz, M. Mette, J. Winden, A. Matzke, M. Matzke (2002)
RNA-directed DNA methylation in ArabidopsisProceedings of the National Academy of Sciences of the United States of America, 99
A. Müller, M. Marins, Y. Kamisugi, P. Meyer (2002)
Analysis of hypermethylation in the RPS element suggests a signal function for short inverted repeats in de novo methylationPlant Molecular Biology, 48
F. Pröls, P. Meyer (1992)
The methylation patterns of chromosomal integration regions influence gene activity of transferred DNA in Petunia hybrida.The Plant journal : for cell and molecular biology, 2 4
J. Schernthaner, M. Matzke, A. Matzke (1988)
Endosperm‐specific activity of a zein gene promoter in transgenic tobacco plantsThe EMBO Journal, 7
X. Fu, A. Kohli, R. Twyman, P. Christou (2000)
Alternative silencing effects involve distinct types of non-spreading cytosine methylation at a three-gene, single-copy transgenic locus in riceMolecular and General Genetics MGG, 263
S. Kumpatla, W. Teng, W. Buchholz, T. Hall (1997)
Epigenetic Transcriptional Silencing and 5-Azacytidine-Mediated Reactivation of a Complex Transgene in Rice, 115
V. Iglesias, E. Moscone, I. Papp, F. Neuhuber, S. Michalowski, T. Phelan, S. Spiker, M. Matzke, A. Matzke (1997)
Molecular and cytogenetic analyses of stably and unstably expressed transgene loci in tobacco.The Plant cell, 9 8
Byeong-ryool Jeong, Dancia Wu-Scharf, Chaomei Zhang, H. Cerutti (2002)
Suppressors of transcriptional transgenic silencing in Chlamydomonas are sensitive to DNA-damaging agents and reactivate transposable elementsProceedings of the National Academy of Sciences of the United States of America, 99
W. Soppe, S. Jacobsen, C. Alonso‐Blanco, J. Jackson, T. Kakutani, M. Koornneef, A. Peeters (2000)
The late flowering phenotype of fwa mutants is caused by gain-of-function epigenetic alleles of a homeodomain gene.Molecular cell, 6 4
M. Matzke, M. Mette, A. Matzke (2000)
Transgene silencing by the host genome defense: implications for the evolution of epigenetic control mechanisms in plants and vertebratesPlant Molecular Biology, 43
S. Altschul, W. Gish, W. Miller, E. Myers, D. Lipman (1990)
Basic local alignment search tool.Journal of molecular biology, 215 3
M. Matzke, M. Primig, J. Trnovsky, A. Matzke (1989)
Reversible methylation and inactivation of marker genes in sequentially transformed tobacco plantsThe EMBO Journal, 8
Z. Avramova, A. Tikhonov, M. Chen, J. Bennetzen (1998)
Matrix attachment regions and structural colinearity in the genomes of two grass species.Nucleic acids research, 26 3
P. Rabinowicz, Kristin Schutz, N. Dedhia, C. Yordán, L. Parnell, L. Stein, W. McCombie, R. Martienssen (1999)
Differential methylation of genes and retrotransposons facilitates shotgun sequencing of the maize genomeNature Genetics, 23
Vicki Vance, H. Vaucheret (2001)
RNA Silencing in Plants--Defense and CounterdefenseScience, 292
V. Vance, H. Vaucheret (2001)
RNA silencing: defense and counterdefenseScience, 292
A. Kovařík, M. Matzke, A. Matzke, B. Koukalová (2001)
Transposition of IS10 from the host Escherichia coli genome to a plasmid may lead to cloning artefactsMolecular Genetics and Genomics, 266
O. Voinnet (2001)
RNA silencing as a plant immune system against viruses.Trends in genetics : TIG, 17 8
M. Wassenegger (2000)
RNA-directed DNA methylationPlantMol. Biol., 43
M. Matzke, A. Matzke, G. Pruss, V. Vance (2001)
RNA-based silencing strategies in plants.Current opinion in genetics & development, 11 2
J. Jakowitsch, I. Papp, E. Moscone, J. Winden, M. Matzke, A. Matzke (1999)
Molecular and cytogenetic characterization of a transgene locus that induces silencing and methylation of homologous promoters in trans.The Plant journal : for cell and molecular biology, 17 2
M. Lohuis, A. Müller, I. Heidmann, I. Niedenhof, P. Meyer (1995)
A repetitive DNA fragment carrying a hot spot for de novo DNA methylation enhances expression variegation in tobacco and petunia.The Plant journal : for cell and molecular biology, 8 6
M. Wassenegger (2000)
RNA-directed DNA methylationPlant Molecular Biology, 43
M. Matzke, M. Mette, J. Jakowitsch, T. Kanno, E. Moscone, J. Winden, A. Matzke (2001)
A test for transvection in plants: DNA pairing may lead to trans-activation or silencing of complex heteroalleles in tobacco.Genetics, 158 1
T. Sijen, I. Vijn, A. Rebocho, R. Blokland, D. Roelofs, J. Mol, J. Kooter (2001)
Transcriptional and posttranscriptional gene silencing are mechanistically relatedCurrent Biology, 11
C. Day, Elsa Lee, Janell Kobayashi, L. Holappa, H. Albert, D. Ow (2000)
Transgene integration into the same chromosome location can produce alleles that express at a predictable level, or alleles that are differentially silenced.Genes & development, 14 22
To test the influence of a Nicotiana tomentosiformis repetitive sequence (R8.3) on transgene expression in N. sylvestris and in N. sylvestris-N. tomentosiformis hybrids, the R8.3 sequence was placed upstream of a nopaline synthase promoter (NOSpro)-NPTII reporter gene in a T-DNA construct. A number of transgenic N. sylvestris lines were produced and in most, the NPTII gene was expressed. In one line, however, the NPTII gene became silenced and methylated in the NOSpro region. The silenced locus was able to trans-inactivate and induce methylation of two stably expressed transgene loci comprising a similar construct. Nucleotide sequence analyses of the three transgene loci revealed that they each contained a single incomplete copy of the T-DNA, which had sustained deletions of varying sizes in the R8.3 region. Paradoxically, the R8.3 DNA upstream of the two active, unmethylated NOSpro-NPTII genes was highly methylated, whereas the R8.3 DNA upstream of the silenced, methylated NOSpro-NPTII gene was less methylated. The methylated portions of the R8.3 sequence corresponded to retroelement remnants. An active NOSpro-NPTII gene downstream of a nearly intact R8.3 sequence did not become methylated in N. sylvestris-N. tomentosiformis hybrids. Thus, methylation in the R8.3 sequence did not spread into adjoining transgene promoters and the effect of the R8.3 dispersed repeat family on transgene expression was negligible. The silencing phenomena observed with the three single-copy transgene loci are discussed in the context of other possible triggers of silencing.
Plant Molecular Biology – Springer Journals
Published: Oct 7, 2004
Access the full text.
Sign up today, get DeepDyve free for 14 days.