Structural modeling of NAD+ binding modes to PARP-1

Structural modeling of NAD+ binding modes to PARP-1 The nuclear protein poly (ADP-ribose) polymerase-1 (PARP-1) plays an important role in the signaling and repair of DNA. PARP-1 catalyses the covalent binding of poly (ADP-ribose) polymers to its subunit, as well as to other acceptor proteins, using NAD+ as a donor of ADP-ribose. Inhibitors of poly (ADP-ribose) polymerase have been shown to be effective in improving radiation therapy and chemotherapy of cancer in clinical testing. Development of new poly (ADP-ribose) polymerase-1 inhibitors based on derivatives of natural compounds such as NAD+ represents a novel and promising strategy. The structure of the complex of human poly (ADP-ribose) polymerase-1 with NAD+ can be a starting point for the rational design of small molecule inhibitors based on NAD+ derivatives. Moreover, there is no crystal structure of the complex of poly (ADP-ribose) polymerase-1 with nicotinamide adenine dinucleotide (NAD+) available yet. In this work, using molecular modeling approaches, we have predicted NAD+ binding modes to PARP-1 at the donor binding site of the catalytic domain. Using structures of PARP-1 homologs in a complex with NAD+, we predict the pharmacophore restraints of NAD+ binding to PARP-1. Based on the clustering of PARP-1 conformations in a complex with cocrystallized inhibitors and the predicted pharmacophore restraints, we propose several possible models of NAD+ binding to PARP-1 at the donor binding site of the catalytic domain. According to the predicted models, two conformations for the pyrophosphate group of NAD+ in complex with PARP-1 at the donor binding site are possible. The proposed models of NAD+ binding to PARP-1 can be validated by the quantitative structure–activity analysis of NAD+ derivatives. We designed two NAD+ derivatives, which can be used to validate the predicted NAD+ binding models. Russian Journal of Genetics: Applied Research Springer Journals

Structural modeling of NAD+ binding modes to PARP-1

Loading next page...
Pleiades Publishing
Copyright © 2017 by Pleiades Publishing, Ltd.
Biomedicine; Human Genetics
Publisher site
See Article on Publisher Site


You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial