Stimulation of Wheat Germ Agglutinin Gene Expression in Root Seedlings by 24-Epibrassinolide

Stimulation of Wheat Germ Agglutinin Gene Expression in Root Seedlings by 24-Epibrassinolide The effects of 24-epibrassinolide (EB) on the growth of four-day-old seedlings of Triticum aestivum L. roots, the accumulation of wheat germ agglutinin (WGA) in them, and its gene expression were studied. EB stimulated growth with two optimum concentrations, 0.4 nM and 0.4 μM. Both concentrations enhanced WGA accumulation in roots, which resulted from the stimulation of its gene expression. The latter was shown by dot-blot hybridization of transcripts with a DNA probe corresponding to WGA. Since EB did not affect the level of endogenous ABA, its direct effect on WGA-gene transcription in wheat roots is suggested. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Stimulation of Wheat Germ Agglutinin Gene Expression in Root Seedlings by 24-Epibrassinolide

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Publisher
Springer Journals
Copyright
Copyright © 2002 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1023/A:1014805724231
Publisher site
See Article on Publisher Site

Abstract

The effects of 24-epibrassinolide (EB) on the growth of four-day-old seedlings of Triticum aestivum L. roots, the accumulation of wheat germ agglutinin (WGA) in them, and its gene expression were studied. EB stimulated growth with two optimum concentrations, 0.4 nM and 0.4 μM. Both concentrations enhanced WGA accumulation in roots, which resulted from the stimulation of its gene expression. The latter was shown by dot-blot hybridization of transcripts with a DNA probe corresponding to WGA. Since EB did not affect the level of endogenous ABA, its direct effect on WGA-gene transcription in wheat roots is suggested.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 13, 2004

References

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