The effects of 24-epibrassinolide (EB) on the growth of four-day-old seedlings of Triticum aestivum L. roots, the accumulation of wheat germ agglutinin (WGA) in them, and its gene expression were studied. EB stimulated growth with two optimum concentrations, 0.4 nM and 0.4 μM. Both concentrations enhanced WGA accumulation in roots, which resulted from the stimulation of its gene expression. The latter was shown by dot-blot hybridization of transcripts with a DNA probe corresponding to WGA. Since EB did not affect the level of endogenous ABA, its direct effect on WGA-gene transcription in wheat roots is suggested.
Russian Journal of Plant Physiology – Springer Journals
Published: Oct 13, 2004
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