Stimulation of type I DNA topoisomerase gene expression by pseudorabies virus

Stimulation of type I DNA topoisomerase gene expression by pseudorabies virus Previous results from our laboratory have demonstrated that type I DNA topoisomerase activity is required for the replication and gene expression of pseudorabies virus (PRV). In the present report, we further analyzed the expression of topoisomerase I in PRV-infected cells, and the western blot result showed that the expression of topoisomerase I was increased after virus infection. The increase sustained to late time of infection when the cytopathic effect was obvious and the synthesis of most host proteins was shut off by PRV. From transient expression assay, it was also found that the promoter of cellular topoisomerase I gene could be stimulated by immediate-early protein (IE180) and viral early protein 0 (EP0), and these two regulatory proteins appeared to work synergistically. Collectively, these findings provide evidence that PRV can stimulate the expression of topoisomerase I and that the stimulation is mediated at least by IE180 and EP0 proteins of PRV at the transcriptional level. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Stimulation of type I DNA topoisomerase gene expression by pseudorabies virus

Loading next page...
 
/lp/springer_journal/stimulation-of-type-i-dna-topoisomerase-gene-expression-by-chJUWDoKwj
Publisher
Springer-Verlag
Copyright
Copyright © Wien by 1997 Springer-Verlag/
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s007050050228
Publisher site
See Article on Publisher Site

Abstract

Previous results from our laboratory have demonstrated that type I DNA topoisomerase activity is required for the replication and gene expression of pseudorabies virus (PRV). In the present report, we further analyzed the expression of topoisomerase I in PRV-infected cells, and the western blot result showed that the expression of topoisomerase I was increased after virus infection. The increase sustained to late time of infection when the cytopathic effect was obvious and the synthesis of most host proteins was shut off by PRV. From transient expression assay, it was also found that the promoter of cellular topoisomerase I gene could be stimulated by immediate-early protein (IE180) and viral early protein 0 (EP0), and these two regulatory proteins appeared to work synergistically. Collectively, these findings provide evidence that PRV can stimulate the expression of topoisomerase I and that the stimulation is mediated at least by IE180 and EP0 proteins of PRV at the transcriptional level.

Journal

Archives of VirologySpringer Journals

Published: Nov 1, 1997

There are no references for this article.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off