Stimulation of in vitro oocyte ovulation by progesterone and homologous pituitary gonadotropic hormone in sturgeons

Stimulation of in vitro oocyte ovulation by progesterone and homologous pituitary gonadotropic... We showed that the percentage of sturgeon oocytes ovulating in vitro in Ringer solution modified for sturgeons (RMS) considerably depends on the concentration of sodium bicarbonate and the concentration of progesterone. Under optimal conditions (0.5 g/L of sodium bicarbonate and 30 ng/mL of progesterone), it can be higher than 80. Oocytes that matured and ovulated under such conditions are capable of normal development. In the best case, approximately 70% of developing embryos (of the number of ovulated oocytes) reach the stage of hatching (dead-line of observation). This method of producing offspring based on the insemination of oocytes that have matured and ovulated in vitro can be used in work with single females of rare and disappearing sturgeon species. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Developmental Biology Springer Journals

Stimulation of in vitro oocyte ovulation by progesterone and homologous pituitary gonadotropic hormone in sturgeons

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Publisher
Springer Journals
Copyright
Copyright © 2012 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Animal Anatomy / Morphology / Histology; Developmental Biology
ISSN
1062-3604
eISSN
1608-3326
D.O.I.
10.1134/S1062360412030083
Publisher site
See Article on Publisher Site

Abstract

We showed that the percentage of sturgeon oocytes ovulating in vitro in Ringer solution modified for sturgeons (RMS) considerably depends on the concentration of sodium bicarbonate and the concentration of progesterone. Under optimal conditions (0.5 g/L of sodium bicarbonate and 30 ng/mL of progesterone), it can be higher than 80. Oocytes that matured and ovulated under such conditions are capable of normal development. In the best case, approximately 70% of developing embryos (of the number of ovulated oocytes) reach the stage of hatching (dead-line of observation). This method of producing offspring based on the insemination of oocytes that have matured and ovulated in vitro can be used in work with single females of rare and disappearing sturgeon species.

Journal

Russian Journal of Developmental BiologySpringer Journals

Published: May 14, 2012

References

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