ISSN 10214437, Russian Journal of Plant Physiology, 2011, Vol. 58, No. 5, pp. 921–927. © Pleiades Publishing, Ltd., 2011.
Original Russian Text © L.A. Volkova, V.V. Urmantseva, A.B. Burgutin, S.N. Maevskaya, A.M. Nosov, 2011, published in Fiziologiya Rastenii, 2011, Vol. 58, No. 5, pp. 766–773.
The methods based not on pathogen elimination
(pesticide application) but on the increase in the plant
immune potential are currently actively developing.
Such methods are primarily related to the usage of
elicitors, inducers of natural resistance. In this case, a
development of relative resistance is determined by
expression of some defense genes; such resistance is
unspecific and inherent in all plant tissues [1, 2]. Since
sometimes elicitors could not provide for a sufficient
level of resistance, some attempts to improve their
defensive action are undertaken in recent years .
Furastanol glycosides (FG) isolated from cell cul
belong to natural com
pounds stimulating plant adaptive mechanisms. In
animals, these compounds manifest immunomodu
lating, anabolic, hepatoprotective, and ovulation
stimulating activities . In the potato cell culture, it
has been demonstrated that low FG concentrations
could function as elicitors capable of the improvement
of plant cell tolerance to oxidative stress. In callus cul
ture, FG changed pigment composition, activated
antioxidant enzymes and recovery systems, which
resulted in the suppression of lipid peroxidation
(POL) and an increase in the potato cell survival .
For intact tomato plants, protective FG effects under
conditions of biotic stress were shown . The effects
of these compounds on plants under conditions of abi
otic stress were not essentially studied.
It was reported that FG, comprising movable
hydrogen atom in the hemiketal hydroxyl group at C22,
in the aqueous solution manifested properties of stable
radicals, which could suppress freeradical processes
. At the same time, their possible antiradical prop
erties toward oxygen anion radical and hydroxyl radi
cal are not studied.
To create oxidative stress in plants, abiotic factors
are usually used, for example, paraquat or hypother
mia. Cell injury under such treatments is largely deter
mined by generation of reactive oxygen species (ROS),
which damage membranes due to POL initiation .
POL activation is considered one of the main units in
the mechanism of stress development [7, 8]. Some
authors indicate that POL products and ROS could
function also as signal molecules providing for the
expression of redoxresponsive genes, many of which
are required for protection against oxidative stress
Stimulation of Defense Responses of In Vitro Potato Plants
by Treatment with Steroid Glycosides under Abiotic Stresses
L. A. Volkova, V. V. Urmantseva, A. B. Burgutin, S. N. Maevskaya, and A. M. Nosov
Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127276 Russia;
fax: 7 (499) 9778018; email: firstname.lastname@example.org
Received May 13, 2010
—To elucidate the biological activity of furastanol glycosides (FG) as elicitors and their possible
action mechanisms, antiradical properties of these compounds and their influence on in vitro potato
L.) plants under abiotic stresses were studied. FG stimulated adaptive responses related
to the activation of guaiacoldependent peroxidase and suppression of lipid peroxidation (POL). Under nor
mal conditions, in 30 min after plant treatment with 4.5
M FG, the level of POL was decreased, activities
of guaiacol and ascorbatedependent peroxidases increased, and activities of catalase and superoxide dismu
tase (SOD) remained unchanged. In 3 days after treatment with FG, POL was decreased by 20–60% in the
leaves and by 50% in the roots. Under stress conditions (paraquat treatment for 11 days or hypothermia for
28 days), FG treatment reduced the level of POL by 20–40% as compared to the effects of stressors alone,
which indicates FG elicitor properties. Antiradical properties of FG toward the hydroxyl radical were mani
fested in the reaction of deoxyDribose destruction in water medium (without homogenate) within the con
centration range from 4.5 to 65
M; the effects toward the oxygen anion radical were insignificant. It is sug
gested that FG influence on generation of reactive oxygen species mediates the stimulation of potato plant
defense responses to environmental factors.
Keywords: Solanum tuberosum
, in vitro plants, furastanol glycosides, paraquat, hypothermia, lipid peroxida
tion, peroxidase, superoxide dismutase, reactive oxygen species, antiradical properties.
: FG—furastanol glycosides; POL—peroxidation of
lipids; SOD—superoxide dismutase; TBARS—thiobarbituric