STENOFOLIA gene and regulation of somatic embryogenesis in Medicago truncatula

STENOFOLIA gene and regulation of somatic embryogenesis in Medicago truncatula Expression of STENOFOLIA (STF) gene was analyzed in different organs of line R-108 barrel medic plants (Medicago truncatula Gaertn.). Maximum levels of STF expression were observed in the generative organs, fruits and flowers, suggesting its involvement in the reproduction process. The local analysis of STF expression during somatic embryogenesis in M. truncatula showed that the STF promoter is activated directly in somatic embryos. Overexpression of STF gene in the calli of R-108 line leads to increased embryogenicity, accompanied by reduced levels of MtGH3.6 gene expression, which is responsible for auxin metabolism, and also reduced expression levels of gene encoding MtHB1 transcription factor. Comparative analysis of MtGH3.6 and MtHB1 gene expression dynamics in the calli of embryogenic 2HA line and non-embryogenic A17 line of M. truncatula showed that these genes demonstrated reduced level of expression in embryogenic calli compared to non-embryogenic. These data suggested that STF stimulates the formation of somatic embryos, directly or indirectly inhibiting the transcription of MtGH3.6, MtHB1 and, probably, many other genes. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

STENOFOLIA gene and regulation of somatic embryogenesis in Medicago truncatula

Loading next page...
 
/lp/springer_journal/stenofolia-gene-and-regulation-of-somatic-embryogenesis-in-medicago-Wu9M0DdtyM
Publisher
Springer Journals
Copyright
Copyright © 2016 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443716060133
Publisher site
See Article on Publisher Site

Abstract

Expression of STENOFOLIA (STF) gene was analyzed in different organs of line R-108 barrel medic plants (Medicago truncatula Gaertn.). Maximum levels of STF expression were observed in the generative organs, fruits and flowers, suggesting its involvement in the reproduction process. The local analysis of STF expression during somatic embryogenesis in M. truncatula showed that the STF promoter is activated directly in somatic embryos. Overexpression of STF gene in the calli of R-108 line leads to increased embryogenicity, accompanied by reduced levels of MtGH3.6 gene expression, which is responsible for auxin metabolism, and also reduced expression levels of gene encoding MtHB1 transcription factor. Comparative analysis of MtGH3.6 and MtHB1 gene expression dynamics in the calli of embryogenic 2HA line and non-embryogenic A17 line of M. truncatula showed that these genes demonstrated reduced level of expression in embryogenic calli compared to non-embryogenic. These data suggested that STF stimulates the formation of somatic embryos, directly or indirectly inhibiting the transcription of MtGH3.6, MtHB1 and, probably, many other genes.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 19, 2016

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off