ISSN 1063-0740, Russian Journal of Marine Biology, 2009, Vol. 35, No. 3, pp. 236–241. © Pleiades Publishing, Ltd., 2009.
Original Russian Text © A.L. Drozdov, S.N. Sharina, S.A. Tyurin, 2009, published in Biologiya Morya.
Bivalves, as a rule, have external insemination, and
tail sperm are typical of them. However, within the limits
of this general pattern of structure, the morphology of
bivalve sperm varies. Some families of the Bivalvia are
characterized by certain variants of external sperm mor-
phology. Formerly studied Paciﬁc species of the Pec-
tinidae and the Mytilidae and Veneridae families had the
same patterns of sperm structure as their kin species from
the Azov, Black and Caspian Seas [3, 7]. The sperm mor-
phology of Bivalvia may be applied in attribution of the
taxonomic status, not only of individual species, but also
of taxa at the family or subfamily rank [2, 4, 9, 12, 14].
Of 20000 of bivalve species, more than 200 inhabit
the Sea of Japan. The ultrastructure of the sperm of spe-
cies of six families from four orders of the Autobranchia
Superorder was described in the present paper, and
sperm of Anomiidae and Trapezidae species were
described for the ﬁrst time. We investigated sperm of for-
(Ostreidae), , to
compare the sperm of that species from different regions.
MATERIAL AND METHODS
Spermatozoa of seven species of bivalves from six
various families (the taxonomy is given after 19), the
Arcoida Order, Arcidae Family—
seaume, 1894 and
1867); the Ostreoida Order, Anomiidae Family—
(Deshayes, 1839), Ostre-
(Thunberg, 1793); the
Veneroida Order, Trapezidae Family—
(Reeve, 1843), Tellinidae Family—
Makiyama, 1927; the Myoida Order, Myidae Fam-
Jay, 1856, were studied.
A. broughtonii, A. boucardi
pled in the Vostok Bay, Sea of Japan.
were sampled in the
Amursky Bay, Sea of Japan.
pled in the northern part of the Peter the Great Bay dur-
ing 24-th cruise of the R/V
. Pieces of
male gonads for ultrastructure research of spermatozoa
were ﬁxed in 2.5% glutaraldehyde in 0.5 M cacodylate
buffer with the addition of sodium chloride up to the
tonicity of sea water. The material was postﬁxed with
on cacodylate buffer and dehydrated by stan-
dard technique in a series of alcohol solutions, then it
was embedded in an Epon–Araldite mix. The ultrathin
sections were cut with an Ultracut ultratome (Reichert).
Sections of silver and gold color were selected for
research. The sections were sequentially stained with
uranyl acetate and lead citrate and examined in a
JEM-100B electron microscope (Jeol).
Sperm Ultrastructure in Representatives of Six Bivalve Families
from Peter the Great Bay, Sea of Japan
A. L. Drozdov
, S. N. Sharina
, and S. A. Tyurin
Institute of Marine Biology, Far East Division, Russian Academy of Sciences, Vladivostok, 690041 Russia;
Academy of Ecology, Marine Biology and Biotechnology, Far Eastern State University, Vladivostok, 690000 Russia
Received January 29, 2009
—The ultrastructure of sperm in seven species of bivalves, the representatives of six families, Arcidae
Anadara broughtonii, Arca boucardi
), Anomiidae (
), Tellinidae (
), Ostreidae (
), Myidae (
) and Trapezidae (
) is described.
All the studied sperm were typical tail sperm, adapted to external insemination, which, however, had a speciﬁc
structure. Differences were revealed in the form of head, acrosome structure and number of mitochondria. The
studied species of the above families had their speciﬁc morphology, the Arcidae species had a bullet- or barrel-
shaped head with four or ﬁve mitochondria in the middle part; the Anomiidae had conic head, the acrosome
with periacrosome material and four mitochondria (a basic feature of sperm is the axial core entering peria-
crosome material and consisting of bundle of actin ﬁlaments); the Myidae had a curved conic head and four
mitochondria; in the Tellinidae the head was bullet-shaped, the periacrosome material contained a ﬁbril com-
ponent and four mitochondria; the Trapezidae had sperm of a conic form with spherical acrosome. The spherical
were similar to sperm of
, but with some
distinctions in the acrosome substructure. The morphology of sperm testiﬁed to the correct attribution of the
Crassostreidae family as a synonym to the Ostreidae family.
acrosome, spermatozoa, bivalves, ultrastructure.