Spectroscopic analysis, docking and molecular dynamics simulation of the interaction of cinnamaldehyde with human serum albumin

Spectroscopic analysis, docking and molecular dynamics simulation of the interaction of... Human serum albumin (HSA) is one of the blood plasma proteins, which plays an important role in the transportation of various natural and pharmaceutical compounds in the body. Cinnamaldehyde is the main component of cinnamon extract which has several medicinal properties. In this study, the conformational changes of HSA in the presence of cinnamaldehyde were investigated by UV–Vis, fluorescence and FT-IR spectroscopic methods and the binding parameters of cinnamaldehyde to HSA were determined. The binding site and driving forces of this interaction were calculated using molecular docking softwares. On the other hand, molecular dynamics simulations were also performed with the GROMACS program package. 4 −1 Using the results of the intrinsic fluorescence spectroscopy data, binding constant of 1.14 × 10  M and number of binding sites 0.883 was obtained. FT-IR measurements have shown that the secondary structure of the protein have been changed by the interaction of cinnamaldehyde with HSA. The free energy of binding (ΔG°) and binding constant (K ) obtained from −1 4 −1 the intrinsic fluorescence results were − 5.54 kcal mol and 1.14 × 10  M respectively, which is in good agreement with molecular docking results. On the other hand, the molecular docking calculations showed that cinnamaldehyde http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Inclusion Phenomena and Macrocyclic Chemistry Springer Journals

Spectroscopic analysis, docking and molecular dynamics simulation of the interaction of cinnamaldehyde with human serum albumin

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Publisher
Springer Netherlands
Copyright
Copyright © 2018 by Springer Science+Business Media B.V., part of Springer Nature
Subject
Chemistry; Organic Chemistry; Food Science; Crystallography and Scattering Methods
ISSN
1388-3127
eISSN
1573-1111
D.O.I.
10.1007/s10847-018-0811-3
Publisher site
See Article on Publisher Site

Abstract

Human serum albumin (HSA) is one of the blood plasma proteins, which plays an important role in the transportation of various natural and pharmaceutical compounds in the body. Cinnamaldehyde is the main component of cinnamon extract which has several medicinal properties. In this study, the conformational changes of HSA in the presence of cinnamaldehyde were investigated by UV–Vis, fluorescence and FT-IR spectroscopic methods and the binding parameters of cinnamaldehyde to HSA were determined. The binding site and driving forces of this interaction were calculated using molecular docking softwares. On the other hand, molecular dynamics simulations were also performed with the GROMACS program package. 4 −1 Using the results of the intrinsic fluorescence spectroscopy data, binding constant of 1.14 × 10  M and number of binding sites 0.883 was obtained. FT-IR measurements have shown that the secondary structure of the protein have been changed by the interaction of cinnamaldehyde with HSA. The free energy of binding (ΔG°) and binding constant (K ) obtained from −1 4 −1 the intrinsic fluorescence results were − 5.54 kcal mol and 1.14 × 10  M respectively, which is in good agreement with molecular docking results. On the other hand, the molecular docking calculations showed that cinnamaldehyde

Journal

Journal of Inclusion Phenomena and Macrocyclic ChemistrySpringer Journals

Published: May 30, 2018

References

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