ISSN 1022-7954, Russian Journal of Genetics, 2007, Vol. 43, No. 11, pp. 1221–1226. © Pleiades Publishing, Inc., 2007.
Original Russian Text © A.A. Slavokhotova, E.N. Andreeva, A.N. Shijan, T.I. Odintsova, V.A. Pukhalskij, 2007, published in Genetika, 2007, Vol. 43, No. 11, pp. 1461–1467.
The cucumber green mottle mosaic virus
(CGMMV) causes one of the most common diseases in
the family Cucurbitaceae. CGMMV belongs to RNA
tobamoviruses; its rigid rodlike virus particle is 300
18 nm in dimensions . CGMMV infection causes
yellow, green, or white mosaic not only on leaves ,
but also on fruits, impairing the fruit quality and yield.
A loss of yield because of this disease can reach 40%.
CGMMV is widespread worldwide and has been
studied in many countries: China, Korea , Japan ,
India , Greece , the United Kingdom , Pakistan
, and France . Cucumber infection with CGMMV
is similarly frequent in Russia. CGMMV was intensely
studied in Russia for a certain period. However, the
genomes of Russian isolates have not been sequenced
so far, while such data are of immense importance for
studying the relationships of these isolates with known
strains, isolated in other countries.
Along with studies of the pathogenic forms of the
virus, it is necessary to obtain attenuated strains, which
accumulate in plants to a lower content, cause no visi-
ble signs, and induce immunity to related pathogenic
strains. The use of such vaccine strains is an effective
means of plant protection. In tobamoviruses, attenuated
strains L11A , ToMV-K , and V69  are used
to protect tomato plants from tobacco mosaic virus
(TMV) tomato strains (tomato mosaic virus (ToMV));
strains M  and V36  protect tobacco plants from
TMV; and strain Tpa18ch  of the pepper mild mot-
tle virus (PMMoV) protects pepper plants from patho-
genic strains of PMMoV. An attenuated CGMMV
strain has been used to vaccinate melon plants .
However, only a few works focus on the construction of
vaccine CGMMV strains to protect cucumber plants
from virus epiphytotics. Complete genome sequencing
in attenuated and related pathogenic strains makes it
possible to deduce the amino acid sequences of virus
proteins; their comparisons reveal the mutations that
are responsible for attenuation or enhanced pathogenic-
ity of the virus.
In this work, we studied the primary structure of the
coat protein (CP) gene in pathogenic (MS-1)
cine (VIROG-43M) CGMMV strains, which were pre-
viously isolated in our laboratory. In tobamoviruses,
CP is the only structural protein responsible for virus
RNA packing, generation of virus particles, virus move-
ment through the plant, and, consequently, development of
systemic infection [15, 16]. In addition, CP determines the
host speciﬁcity . CP is the best studied CGMMV pro-
tein: GenBank contains data on CP for 15 CGMMV
strains, on the movement protein for 10 strains, and on the
total genome for 5 strains.
Serological analysis of CP was the main method in
early studies of the pathogenic and attenuated
CGMMV strains in our laboratory. Hence, in studies of
the genome structure, it was of interest ﬁrst and fore-
most to sequence the CP genes of these strains and to
compare the deduced CP amino acid sequences with
those of other tobamoviruses and CGMMV strains iso-
lated in various countries worldwide.
Specifics of the Coat Protein Gene in Russian Strains
of the Cucumber Green Mottle Mosaic Virus
A. A. Slavokhotova, E. N. Andreeva, A. N. Shijan, T. I. Odintsova, and V. A. Pukhalskij
Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991 Russia;
fax: (495) 132-89-62; e-mail: email@example.com
Received February 6, 2007
—The primary structure of the coat protein (CP) gene was examined for pathogenic strain MS-1
vaccine strain VIROG-43M of the cucumber green mottle mosaic virus (CGMMV). In CP amino acid compo-
sition, strains MS-1 and VIROG-43M are typical representatives of CGMMV: their CPs have 98–100% homol-
ogy to CPs of other tobamoviruses of the group. The CP gene has the same nucleotide composition in patho-
genic MS-1 and vaccine VIROG-43M, indicating that strain attenuation is not determined by this gene. The CP
amino acid sequences of the two Russian strains are fully identical to the CP sequences of two Greek strains,
GR-3 and GR-5. However, the nucleotide sequences of their genes differ in 13 bp, testifying to the difference
between the Russian and Greek strains.
The nucleotide sequence of the CP gene of CGMMV strain MS-1
has been deposited in GenBank under accession no. EF521882.