ISSN 1022-7954, Russian Journal of Genetics, 2007, Vol. 43, No. 4, pp. 404–410. © Pleiades Publishing, Inc., 2007.
Original Russian Text © Sh.Yu. Yunuskhanov, D.Yu. Ataev, I.Zh. Kurbanbaev, 2007, published in Genetika, 2007, Vol. 43, No. 4, pp. 508–515.
At present, about 50 cotton species are recognized.
They include diploids and natural amphidiploids .
Cotton industry is based on two amphidiploid species:
L., yielding about 90% of global
cotton ﬁber production, and
5% . Other cotton species, both diploid and amphid-
iploid, are involved in breeding programs as donors of
commercially valuable traits: early ripening, pest resis-
tance, and characters improving ﬁber quality.
Modern biology is characterized by intensiﬁcation
of studies on molecular markers linked to commercially
valuable traits of a plant species. Such markers include
polymorphism of DNA fragments [3–5] and polymor-
phism of proteins and isoenzymes [6–9]. Application of
protein electrophoresis to studying genetic relation-
ships and to solution of taxonomic and phylogenetic
questions at the molecular level has been illustrated by
many authors [10–12]. It is believed that protein pat-
terns of seeds are stable and little variable in the course
of evolution. It should be noted that protein and isoen-
zyme markers are more readily available and less
expensive than DNA fragment analysis.
Comparison of protein patterns of seeds of various
cotton species and varieties allows detection of some
protein markers and establishment of their relationships
with some valuable characters [13–15].
Detection of protein markers in diploid species, par-
ticularly, in ancestors of modern amphidiploids, would
allow better understanding of cotton phylogeny. These
markers can be applied to identiﬁcation of varieties and
species. Study of the linkage of these markers with
commercially valuable traits is important for their use
in marker-associated cotton breeding.
In this study, we compare SDS electrophoretic spec-
tra of water-soluble, buffer-soluble, and hard-soluble
proteins from seeds of cotton diploid species of genome
groups A and D and some natural amphidiploid species.
MATERIALS AND METHODS
Experiments were performed with seeds of cotton
species obtained form the collection of the Laboratory
of Cotton Systematics and Introduction, Institute of
Plant Genetics and Experimental Biology, Academy of
Sciences of Uzbekistan, and from the collection of the
Zaitsev Institute of Cotton Breeding and Seed Produc-
tion. Diploid species:
Kell (genome D).
Natural amphidiploid species:
isolation from seeds and electrophoretic analysis of
water-soluble, buffer-soluble, and hard- soluble pro-
teins were performed as described in . SDS electro-
phoresis was performed by the Laemmli method .
The similarity of protein patterns between various cot-
ton species was calculated as
Species-specific Features of the Protein Patterns
of Diploid Cotton Seeds with A and D Genomes
and of Some Amphidiploids
Sh. Yu. Yunuskhanov, D. Yu. Ataev, and I. Zh. Kurbanbaev
Institute of Plant Genetics and Experimental Biology, Academy of Sciences of Uzbekistan,
Yukari-yuz, Tashkent oblast, Uzbekistan fax: (998-712)64-22-30; e-mail: email@example.com
Received February 16, 2006; in ﬁnal form, September 11, 2006
—Differences between species were revealed in electrophoretic patterns of seed proteins of various
diploid cotton species with A and D genomes and some amphidiploids. Reference spectra and electrophoretic
formulas were compiled for representatives of diploid and amphidiploid species, and the electrophoretic spectra
were visually evaluated. They would allow identiﬁcation of various cotton species, varieties, and lines. Homol-
ogy between cotton species was estimated from the results of electrophoretic protein studies. The homology
between species of a single genome group was shown to be closer than between species belonging to different