ISSN 10674136, Russian Journal of Ecology, 2012, Vol. 43, No. 3, pp. 210–217. © Pleiades Publishing, Ltd., 2012.
Original Russian Text © V.P. Perevozkin, A.A. Printseva, S.S. Bondarchuk, M.I. Gordeev, 2012, published in Ekologiya, 2012, No. 3, pp. 188–195.
Mosquitoes of the genus
cidae) attract special attention from biologists, medical
researchers and practitioners, and epidemiological sur
veillance services. These dipterans belong to the com
plex of bloodsucking insects that are a major nuisance to
humans and livestock; they are also important epidemi
ologically as vectors of malaria. At the same time, they
are convenient model organisms for ecological and
population genetic analysis (Kabanova et al., 1972).
The Altai Republic, and Teletskoye Lake area in
particular, are becoming one of the popular tourist
centers of Russia, with the flow of visitors to the region
increasing in summer. This may result in local out
breaks of malaria, since the Altai Republic is in the
zone of potential malaria persistence (Shipitsina,
1964). To take successful measures against this infec
tion, it is important to know the species composition
of mosquitoes and their distribution in the region, as
well as the adaptive inversion structure of their popu
lations (Stegnii, 1991).
Therefore, the purpose of this study was to deter
mine the species composition, population genetic
structure, and ecological specialization of
mosquitoes in the area of Teletskoye Lake.
MATERIAL AND METHODS
This study was performed on fourth instar larvae of
mosquitoes collected in 2007 and 2008 from
various biotopes in the environs of Teletskoye Lake. In
2007 (July 26–29), these were six biotopes located at
the mouths of the rivers Samysh, Koldor, Kamga,
Chulyshman (left bank), and Kyga, and also in
Shchuch’e Lake in the Biya River floodplain, 9 km
from Artybash village. In 2008 (July 26–August 2), in
addition to these biotopes, the larvae were collected
from Karasevoe Lake near the village of Iogach and
the Chulyshman River mouth, on the right bank near
the Karagai Tourist Camp.
The larvae were preserved in an ethanol–acetic
acid mixture (3 : 1) and transported to the laboratory
to make temporary preparations of the salivary gland
polytene chromosomes stained with lactoaceto
orcein (Kabanova et al., 1972). All preparations were
examined for the presence of inracellular parasites
A total of 931 preparations were made and analyzed
over two years: 439 in 2007 and 492 in 2008. The com
parative analysis of the results was performed by the
method (confidence level
= 0.05 in all cases).
The level of karyotypic polymorphism in
was assessed using the diversity index calculated
by the following formula (Perevozkin et al., 2009):
is the maximum frequency (%) found among
possible (or observed) frequencies of karyotypic
classes in the sample.
The water temperature was measured with a porta
ble HANNA Water Test device.
Spatial Distribution and Karyotypic Composition of
Mosquitoes (Diptera, Culicidae) in the Environs of Teletskoye Lake
V. P. Perevozkin
, A. A. Printseva
, S. S. Bondarchuk
, and M. I. Gordeev
Research Institute of Biology and Biophysics, Tomsk State University, pr. Lenina 36, Tomsk, 634050 Russia
Tomsk State Pedagogical University, ul. Kievskaya 60, Tomsk, 634061 Russia
Moscow Regional State University, ul. Radio 10a, Moscow, 105005 Russia
Received February 14, 2011
—The species composition and inversion structure of
micropopulations have been studied
in the environs of Teletskoye Lake, the Altai Republic. Two
have been identified cytogenetically: Their zonal distribution has been determined, reflecting specific cli
matic features in the southern and northern parts of the lake. Intraspecific chromosomal polymorphism has
been revealed in both mosquito species, with the inversion and karyotypic diversity being higher in the south
ern larval populations of
, karyotype, inversion, homozygote, heterozygote, diversity index.