ISSN 1022-7954, Russian Journal of Genetics, 2017, Vol. 53, No. 4, pp. 514–517. © Pleiades Publishing, Inc., 2017.
Original Russian Text © K.V. Boris, A.M. Kudryavtsev, E.Z. Kochieva, 2017, published in Genetika, 2017, Vol. 53, No. 4, pp. 507–511.
Sorbitol-6-Phosphate Dehydrogenase Gene (S6PDH)
Polymorphism in Tribe Pyreae (Rosaceae) Species
K. V. Boris
*, A. M. Kudryavtsev
, and E. Z. Kochieva
Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991 Russia
Institute of Bioengineering, Federal Research Center Fundamentals of Biotechnology,
Russian Academy of Sciences, Moscow, 119071 Russia
Department of Biotechnology, Moscow State University, Moscow, 119234 Russia
Received May 18, 2016
Abstract⎯The sorbitol-6-phosphate dehydrogenase gene (S6PDH) sequences of eight tribe Pyreae species
(Rosaceae) are studied for the first time. The exon–intron structure and polymorphism of the nucleotide and
amino acid sequences of this gene are characterized. The interspecific polymorphism of the S6PDH coding
sequences in the studied Pyreae species is 8.36%. Sorbitol-6-phosphate dehydrogenase gene expression in
S. aucuparia, A. melanocarpa, and M. domestica (cv. Skala) leaves is studied. The highest level of S6PDH
expression is detected in mature leaves.
Keywords: Rosaceae, carbohydrate metabolism genes, sorbitol-6-phosphate dehydrogenase, genetic poly-
Unlike most plants, fruit tree crops of the Rosaceae
family (genera Malus, Pyrus, Sorbus, etc.) synthesize
sorbitol in addition to sucrose during the process of
photosynthesis; sorbitol not only is a source of carbon
for vegetative growth of the plant and fruit develop-
ment but also is involved in response to abiotic stresses
such as salinification of the soil, drought, and low
The main enzyme of sorbitol synthesis is sorbitol-
6-phosphate dehydrogenase (S6PDH), which facili-
tates conversion of glucose 6-phosphate into sorbitol-
6-phosphate in the leaves.
Complete sequences of the S6PDH gene, which
encodes sorbitol-6-phosphate dehydrogenase, are
currently known for apple (Malus domestica) [5, 6] and
a number of wild-growing members of the genus
Malus ; cDNA sequences of this gene are also
known for the members of genera Prunus (NCBI acc.
nos. EF576940, FJ645737) and Pyrus (NCBI acc.
nos. KC506733, XM_009380183).
The highest level of S6PDH expression in peach
(Prunus persica) was demonstrated in mature leaves at
the beginning of summer; it gradually decreases along
with the ripening of the fruits and increases again after
the harvesting . In addition, abscisic acid, high con-
centrations of salt, low temperatures, and drought affect
the S6PDH expression [1, 4]. Transgenic tobacco and
persimmon plants transformed by the apple S6PDH gene
had increased sorbitol contents and thus an increased tol-
erance to osmotic stress [9, 10].
Data on sequences encoding sorbitol-6-phosphate
dehydrogenase in wild-growing members of the tribe
Pyreae (Rosaceae), such as rowan (Sorbus), hawthorn
(Crataegus), and other species related to more thor-
oughly studied genera Malus and Prunus, are currently
The aim of the present study is to determine the
nucleotide sequences of the sorbitol-6-phosphate
dehydrogenase gene in eight members of the tribe
Pyreae (Rosaceae), to assess their variability, and
determine the expression of S6PDH in leaves of the
Eight species of the tribe Pyreae (Rosaceae) used
in the present study were provided by the Michurin
All-Russia Research and Development Institute of
Fruit Crop Genetics and Breeding and the Tsitsin
Main Botanical Garden (Russian Academy of Sci-
ences): Chaenomeles japonica (Maule’s quince), Cydo-
nia oblonga (quince), Mespilus germanica (common
medlar), Sorbus aucuparia (rowan), Aronia melano-
carpa (black chokeberry), Cotoneaster lucidus (shiny
cotoneaster), Pyrus elata (pear), and Crataegus monogyna
(common hawthorn). Total DNA of all samples was
extracted by the CTAB method from fresh leaves .
Previously designed primers were used for cloning
and subsequent sequencing of sorbitol-6-phosphate
dehydrogenase sequences . Amplification of