ISSN 1021-4437, Russian Journal of Plant Physiology, 2009, Vol. 56, No. 1, pp. 133–137. © Pleiades Publishing, Ltd., 2008.
Published in Russian in Fiziologiya Rastenii 2009, Vol. 56, No. 1, pp. 147–151.
is a Chinese traditional medici-
nal plant , the natural resources of which have
decreased due to overharvesting. A clinical experience
for over several thousand years has shown the best
quality of the plants growing in Maoshan area (Jiangsu
province, China). The resources of the wild plants in
Maoshan area have been severely depleted. Wild plants
had also been found everywhere in Xishan area of
Beijing province, but their quality is worse. Acidity of
the soils in Maoshan area (pH 5.3) is stronger than in
Xishan area (pH 6.0) .
The low pH in soil might affect plant growth indi-
rectly by elevating aluminum or manganese solubility
and by limiting an availability of molybdenum, phos-
phorus, calcium, or magnesium. On the other hand, the
low pH can directly inhibit plant growth. Exogenous
ABA has helped to overcome the adverse effects of low
pH on the root growth [3, 4]. Meanwhile, Konno et al.
 have reported that the lettuce seedlings formation of
root hairs was induced by low pH in a mineral medium.
Takahashi et al.  assumed that both auxin and ethyl-
ene had been involved in low-pH induced root hair ini-
tiation. Also jasmonates have been found to promote
the root hair formation in
, possibly through
an interaction with ethylene [7, 8].
plants are rich in some essential oils, up to
7% in dried roots. The major active compounds are ses-
quiterpenes. This group includes
lone, elemol, atractylenolid, atractylodin, and hinesol.
The text was submitted by the authors in English.
-Eudesmol is the most important active compound in
these plants. Environmental factors can greatly inﬂu-
ence emission of volatiles, their yield and composition
of the essential oils [9, 10].
To better understand how the low pH affects growth
plants and accumulation of active com-
pounds, we examined the root growth and
content in seedling roots grown on MS medium at
pH 5.3 as the low pH treatment and pH 6.0 as the control.
MATERIALS AND METHODS
Plant material and culture conditions.
seeds, collected from Hubei province, China,
were immersed in deionized water for 3 days to induce
germination. Seedlings were sterilized by 1% HgCl
and then transferred to the MS basal medium contain-
ing 30 g/l sucrose and 8 g/l agar in bottles, where they
were cultivated for 30 and 60 days at a 16 h-light pho-
. MS media were separately adjusted
to pH 5.3 with HCl and to pH 6.0 with NaOH.
Measurement of root hairs, root length and dry
Ten seedlings per treatment were taken to measure
the number of root hairs in apical segments 2–3 mm
behind the apex. They were counted under stereomicro-
scope (Nikon, Japan) . On 30 and 60 days after
treatment, 10 seedlings per treatment were used to mea-
sure the length of main root by vernier calipers (Mitu-
toyo, Japan). The roots were dried at room temperature
for 3 days and weighed.
ELISA assay for phytohormones.
In ten roots from
30-day-old or 60-day-old seedlings, extraction, puriﬁ-
cation and determination of IAA, GA, ABA, and ZR by
Soil Acidity Elevates Some Phytohormone and
Contents in Roots of
, Y. J. Liu
, L. Q. Huang
, G. H. Cui
, and G. F. Fu
Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China;
fax:+86-10-8404-4340; e-mail: email@example.com
Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Received September 12, 2007
—We examined a low pH effect on root growth of
, a Chinese traditional medici-
nal plant, and accumulation of the major active compound,
-eudesmol. Our results have shown that low pH
increases root hair density and root dry weight, but reduces the length of the main root. More acidic pH stimu-
lates production of
Key words: Atractylodes lancea -
-eudesmol - acidic medium - roots
: ZR—zeatin riboside.