Sodium nitroprusside enhances the elicitation power of methyl jasmonate for ginsenoside production in Panax ginseng roots

Sodium nitroprusside enhances the elicitation power of methyl jasmonate for ginsenoside... The effects of methyl jasmonate (MJ) and sodium nitroprusside (SNP) on ginsenoside production were investigated in ginseng adventitious roots. The role of nitric oxide (NO) production and reactive oxygen species (ROS) was also elucidated. Different concentrations of SNP were applied to ginseng adventitious roots. The highest accumulation of ginsenoside was recorded for 200 μM of SNP. Ginsenoside biosynthesis-related genes were highly induced by 100 μM MJ in combination with 200 μM of SNP compared to MJ alone , which subsequently led to higher ginsenoside accumulation after 72 h. For the first time, a full length NO-associated (NOA) cDNA clone was isolated and characterized from the embryogenic callus of ginseng with predicted localization in the mitochondria. The open reading frame was 1626 bp with a deduced amino acid sequence of 541 residues, which shared a high degree of homology with the NO associated protein from Solanum tuberosum. PgNOA showed higher transcript levels in treated roots with combined MJ and SNP compared to MJ alone. Increased production of NO was observed after addition of SNP to MJ-treated samples. There was no difference in transcript levels for lipoxygenase, a key enzyme in the jasmonic acid pathway in MJ- and SNP-treated roots compared to roots treated with MJ alone. The superoxide dismutase 1 (SOD1) gene was highly responsive to MJ and SNP treatment compared to treatment with MJ alone. SOD1 catalyzes the dismutation of toxic superoxide radicals into either molecular oxygen or hydrogen peroxide (H2O2) resulting in higher production of these products. H2O2 can be subsequently converted to water by the action of ascorbate peroxidase (APX), which was highly expressed in combined treatment with MJ and SNP compared to treatment with MJ alone. The results obtained here suggest that SNP enhanced the elicitation power of MJ for increased ginsenoside accumulation. Moreover, this process was mediated by a complex signal transduction network including NO biosynthesis and ROS generation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Research on Chemical Intermediates Springer Journals

Sodium nitroprusside enhances the elicitation power of methyl jasmonate for ginsenoside production in Panax ginseng roots

Loading next page...
 
/lp/springer_journal/sodium-nitroprusside-enhances-the-elicitation-power-of-methyl-TlvlhLPl0B
Publisher
Springer Journals
Copyright
Copyright © 2015 by Springer Science+Business Media Dordrecht
Subject
Chemistry; Catalysis; Physical Chemistry; Inorganic Chemistry
ISSN
0922-6168
eISSN
1568-5675
D.O.I.
10.1007/s11164-015-2188-x
Publisher site
See Article on Publisher Site

Abstract

The effects of methyl jasmonate (MJ) and sodium nitroprusside (SNP) on ginsenoside production were investigated in ginseng adventitious roots. The role of nitric oxide (NO) production and reactive oxygen species (ROS) was also elucidated. Different concentrations of SNP were applied to ginseng adventitious roots. The highest accumulation of ginsenoside was recorded for 200 μM of SNP. Ginsenoside biosynthesis-related genes were highly induced by 100 μM MJ in combination with 200 μM of SNP compared to MJ alone , which subsequently led to higher ginsenoside accumulation after 72 h. For the first time, a full length NO-associated (NOA) cDNA clone was isolated and characterized from the embryogenic callus of ginseng with predicted localization in the mitochondria. The open reading frame was 1626 bp with a deduced amino acid sequence of 541 residues, which shared a high degree of homology with the NO associated protein from Solanum tuberosum. PgNOA showed higher transcript levels in treated roots with combined MJ and SNP compared to MJ alone. Increased production of NO was observed after addition of SNP to MJ-treated samples. There was no difference in transcript levels for lipoxygenase, a key enzyme in the jasmonic acid pathway in MJ- and SNP-treated roots compared to roots treated with MJ alone. The superoxide dismutase 1 (SOD1) gene was highly responsive to MJ and SNP treatment compared to treatment with MJ alone. SOD1 catalyzes the dismutation of toxic superoxide radicals into either molecular oxygen or hydrogen peroxide (H2O2) resulting in higher production of these products. H2O2 can be subsequently converted to water by the action of ascorbate peroxidase (APX), which was highly expressed in combined treatment with MJ and SNP compared to treatment with MJ alone. The results obtained here suggest that SNP enhanced the elicitation power of MJ for increased ginsenoside accumulation. Moreover, this process was mediated by a complex signal transduction network including NO biosynthesis and ROS generation.

Journal

Research on Chemical IntermediatesSpringer Journals

Published: Aug 19, 2015

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off