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A. Kleinzeller, C. Dodia, A. Chander, A. Fisher (1994)
Na(+)-dependent and Na(+)-independent systems of choline transport by plasma membrane vesicles of A549 cell line.The American journal of physiology, 267 5 Pt 1
A. Fisher, A. Chander, C. Dodia, J. Reicherter, A. Kleinzeller (1989)
Choline transport by lung epithelium.American journal of respiratory cell and molecular biology, 1 6
O. Lowry, N. Rosebrough, A. Farr, R. Randall (1951)
Protein measurement with the Folin phenol reagent.The Journal of biological chemistry, 193 1
H. Yamamura, S. Snyder (1973)
HIGH AFFINITY TRANSPORT OF CHOLINE INTO SYNAPTOSOMES OF RAT BRAIN 1Journal of Neurochemistry, 21
L. Winkle, A. Campione, D. Mann, H. Wasserlauf (1993)
The cation receptor subsite of the choline transporter in preimplantation mouse conceptuses resembles a cation receptor subsite of several amino acid transporters.Biochimica et biophysica acta, 1146 1
D. Wheeler (1979)
A MODEL OF HIGH AFFINITY CHOLINE TRANSPORT IN RAT CORTICAL SYNAPTOSOMESJournal of Neurochemistry, 32
M. Kessler, O. Acuto, C. Storelli, H. Murer, M. Müller, G. Semenza (1978)
A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes. Their use in investigating some properties of D-glucose and choline transport systems.Biochimica et biophysica acta, 506 1
Patrick Schloss, W. Mayser, Heinrich Betz (1994)
The putative rat choline transporter CHOT1 transports creatine and is highly expressed in neural and muscle-rich tissues.Biochemical and biophysical research communications, 198 2
W. Mayser, P. Schloss, H. Betz (1992)
Primary structure and functional expression of a choline transporter expressed in the rat nervous systemFEBS Letters, 305
N. Bertrand, J. Bralet, A. Beley (1990)
Turnover Rate of Brain Acetylcholine Using HPLC Separation of the TransmitterJournal of Neurochemistry, 55
G. Sterling, P. Doukas, F. Ricciardi, D. Biedrzycka, J. O'neill (1986)
Inhibition of High‐Affinity Choline Uptake and Acetylcholine Synthesis by Quinuclidinyl and Hemicholinium DerivativesJournal of Neurochemistry, 46
J. Kerr, A. Fisher, A. Kleinzeller (1981)
Transport of glucose analogues in rat lung.The American journal of physiology, 241 3
R. Bland, C. Boyd (1986)
Cation transport in lung epithelial cells derived from fetal, newborn, and adult rabbits.Journal of applied physiology, 61 2
A. Fisher, C. Dodia, A. Chander, Arnost Kleinzeller (1992)
Transport of choline by plasma membrane vesicles from lung-derived epithelial cells.The American journal of physiology, 263 6 Pt 1
R. Devés, P. Chávez, C. Boyd (1992)
Identification of a new transport system (y+L) in human erythrocytes that recognizes lysine and leucine with high affinity.The Journal of Physiology, 454
I. Ducis, V. Whittaker (1985)
High-affinity, sodium-gradient-dependent transport of choline into vesiculated presynaptic plasma membrane fragments from the electric organ of Torpedo marmorata and reconstitution of the solubilized transporter into liposomes.Biochimica et biophysica acta, 815 1
R. Yost, A. Chander, C. Dodia, A. Fisher (1986)
Synthesis of phosphatidylcholine by rat lung during choline deficiency.Journal of applied physiology, 61 6
Jay Simon, Samir Atweh, Michael Kuhar (1976)
SODIUM‐DEPENDENT HIGH AFFINITY CHOLINE UPTAKE: A REGULATORY STEP IN THE SYNTHESIS OF ACETYLCHOLINEJournal of Neurochemistry, 26
E. Bligh, Dyer W.J.A. (1959)
A rapid method of total lipid extraction and purification.Canadian journal of biochemistry and physiology, 37 8
Richard Porter, John Scott, M. Brand (1992)
Choline transport into rat liver mitochondria. Characterization and kinetics of a specific transporter.The Journal of biological chemistry, 267 21
R. Devés, Sylvia Angelo (1996)
Changes in Membrane and Surface Potential Explain the Opposite Effects of Low Ionic Strength on the Two Lysine Transporters of Human Erythrocytes*The Journal of Biological Chemistry, 271
The uptake of 3H-labeled choline by a suspension of isolated type II epithelial cells from rat lung has been studied in a Ringer medium. Uptake was linear for 4 min at both 0.1 μm and 5.0 μm medium choline; at 5 μm, only 10% of the label was recovered in a lipid fraction. Further experiments were conducted at the low concentration (0.1 μm), permitting characterization of the properties of high-affinity systems. Three fractions of choline uptake were detected: (i) a sodium-dependent system that was totally inhibited by hemicholinium-3 (HC-3); (ii) a sodium-independent uptake, when Na+ was replaced by Li+, K+ or Mg2+, inhibited by HC-3; (iii) a residual portion persisting in the absence of Na+ and unaffected by HC-3. Choline uptake was sigmoidally related to the medium Na+ concentration. Kinetic properties of the uptake of 0.1 μm 3H-choline in the presence and absence of medium Na+ were examined in two ways. (a) Inhibition by increasing concentrations of unlabeled choline (0.5–100 μm) was consistent with the presence of two Michaelis-Menten-type systems in the presence of Na+; a Na+-dependent portion (a mean of 0.52 of the total) had a K m for choline of 1.5 μm while K m in the absence of Na+ (Li+ substituting) was 18.6 μm. (b) Inhibition by HC-3 (0.3–300 μm) gave Ki values of 1.7 μm and 5.0 μm HC-3 for the Na+-dependent and -independent fractions. The apparent K m of the Na+-dependent uptake is lower than that reported previously for lung-derived cells and is in the range of the K m values reported for high-affinity, Na+-dependent choline uptake by neuronal cells.
The Journal of Membrane Biology – Springer Journals
Published: Mar 15, 1998
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