The acidic fibroblast growth factor (aFGF) is a member of FGF family which is composed of 154 amino acids. In order to explore the functional anti-aFGF single-chain variable fragment antibody (scFv) expression in eukaryote system and to enhance the binding activity, the functional scFv specific for human aFGF was expressed in tobacco plants via TMV vector mediated by Agrobacterium. The engineered bacterial EHA105: p35S-30B-scFv was inoculated into the leaves of Nicotiana benthamiana plants by needleless injection. Green fluorescence protein was used as a reporter gene and accumulated at the highest level on the 8th day of post-inoculation. Expression of scFv was identified by RT-PCR and Western-blot. The yield of anti-aFGF-scFv protein was reached to 0.5% of the plant total soluble proteins and a fair binding activity with its antigen aFGF was detected.
Russian Journal of Plant Physiology – Springer Journals
Published: Apr 29, 2015
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