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SINE targeting of bovine microsatellites from bovine/rodent hybrid cell lines

SINE targeting of bovine microsatellites from bovine/rodent hybrid cell lines Mammahan Genome 8, 365-367 (1997). e//o///e 9 Spnnger-Verlag New York lnc 1997 SINE targeting of bovine microsatellites from bovine/rodent hybrid cell lines Silja Kostia, 1 Johanna Vilkki, 2 Minna Pirinen, 1 James E. Womack, 3 William Barendse, 4 Sirkka-Liisa Varvio ] ~Department of Biosciences, Division of Genetics, P.O. Box 56, Viikinkaari 5, FIN-00014 University of Helsinki, Helsinkl, Finland 2Animal Breeding Section, Agricultural Research Centre, 31600 Jokioinen, Finland 3Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas 77643, USA 4CSIRO, Division of Tropical Animal Production, University of Queensland, Brisbane 4072, Australia Received: 22 August 1996 / Accepted: 24 January 1997 Short interspersed elements (SINEs), ubiquitous components of Table 1. Seven SINE hbraries were constructed by two different strategies: either by using fragments from one PCR (cl, c2, rl, and r3) or poohng fragments from three eukaryote genomes (reviewed by Deininger and Batzer 1993), can PCRs (c3, c4, r2). PCR fragment pools were extracted from 1.5% NuSleve GTG provide primers to PCR amplify DNA segments flanked by these low-melting agarose (FMC BioProducts, ME, USA) by the Wizard PCR Preps TM sequences. Species (or family) specificity of SINEs has turned out purificataon system (Promega, Madison, W]s., USA), cloned by using the http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

SINE targeting of bovine microsatellites from bovine/rodent hybrid cell lines

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References (13)

Publisher
Springer Journals
Copyright
Copyright © Springer-Verlag 1997
Subject
Life Sciences; Cell Biology; Anatomy; Zoology
ISSN
0938-8990
eISSN
1432-1777
DOI
10.1007/s003359900443
Publisher site
See Article on Publisher Site

Abstract

Mammahan Genome 8, 365-367 (1997). e//o///e 9 Spnnger-Verlag New York lnc 1997 SINE targeting of bovine microsatellites from bovine/rodent hybrid cell lines Silja Kostia, 1 Johanna Vilkki, 2 Minna Pirinen, 1 James E. Womack, 3 William Barendse, 4 Sirkka-Liisa Varvio ] ~Department of Biosciences, Division of Genetics, P.O. Box 56, Viikinkaari 5, FIN-00014 University of Helsinki, Helsinkl, Finland 2Animal Breeding Section, Agricultural Research Centre, 31600 Jokioinen, Finland 3Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas 77643, USA 4CSIRO, Division of Tropical Animal Production, University of Queensland, Brisbane 4072, Australia Received: 22 August 1996 / Accepted: 24 January 1997 Short interspersed elements (SINEs), ubiquitous components of Table 1. Seven SINE hbraries were constructed by two different strategies: either by using fragments from one PCR (cl, c2, rl, and r3) or poohng fragments from three eukaryote genomes (reviewed by Deininger and Batzer 1993), can PCRs (c3, c4, r2). PCR fragment pools were extracted from 1.5% NuSleve GTG provide primers to PCR amplify DNA segments flanked by these low-melting agarose (FMC BioProducts, ME, USA) by the Wizard PCR Preps TM sequences. Species (or family) specificity of SINEs has turned out purificataon system (Promega, Madison, W]s., USA), cloned by using the

Journal

Mammalian Genome – Springer Journals

Published: May 1, 1997

Keywords: Bovine Genome; Hybrid Cell Line; Synteny Mapping; Sine Primer; Somatic Hybrid Cell Line

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