Mammahan Genome 8, 365-367 (1997). e//o///e 9 Spnnger-Verlag New York lnc 1997 SINE targeting of bovine microsatellites from bovine/rodent hybrid cell lines Silja Kostia, 1 Johanna Vilkki, 2 Minna Pirinen, 1 James E. Womack, 3 William Barendse, 4 Sirkka-Liisa Varvio ] ~Department of Biosciences, Division of Genetics, P.O. Box 56, Viikinkaari 5, FIN-00014 University of Helsinki, Helsinkl, Finland 2Animal Breeding Section, Agricultural Research Centre, 31600 Jokioinen, Finland 3Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas 77643, USA 4CSIRO, Division of Tropical Animal Production, University of Queensland, Brisbane 4072, Australia Received: 22 August 1996 / Accepted: 24 January 1997 Short interspersed elements (SINEs), ubiquitous components of Table 1. Seven SINE hbraries were constructed by two different strategies: either by using fragments from one PCR (cl, c2, rl, and r3) or poohng fragments from three eukaryote genomes (reviewed by Deininger and Batzer 1993), can PCRs (c3, c4, r2). PCR fragment pools were extracted from 1.5% NuSleve GTG provide primers to PCR amplify DNA segments flanked by these low-melting agarose (FMC BioProducts, ME, USA) by the Wizard PCR Preps TM sequences. Species (or family) specificity of SINEs has turned out purificataon system (Promega, Madison, W]s., USA), cloned by using the
Mammalian Genome – Springer Journals
Published: Mar 21, 2009
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