Three fractions were isolated from Parmelia sulcata lichen thalli: mycobiont-enriched fraction, highly purified preparation of Trebouxia sp. alga, and a photobiont-enriched fraction, in which the fungal component and symbiotic relations between the bionts were retained. The ratio between the fungal and algal components in all fractions was estimated from the photosynthesis/respiration and chlorophyll/dry weight ratios. Simple methods for lichen thallus fractionation are suggested to study symbiotic relationships of bionts.
Russian Journal of Plant Physiology – Springer Journals
Published: Apr 7, 2005
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