Leaf samples of Siegesbeckia glabrescens showing yellow vein, enation, and stunting symptoms were collected in Guangdong province, China. A specific 500-bp product was consistently detected in total DNA extracts, amplified with universal primers specific for members of the genus Begomovirus . Comparison of partial DNA sequences revealed that these virus isolates were identical, and therefore isolates GD13, GD24 and GD27 were selected for further sequence analysis. The complete nucleotide sequences of GD13, GD24 and GD27 were all found to be 2768 nucleotides (nts) long, with two open reading frames (ORFs) in the virion-sense strand and four ORFs in the complementary-sense strand, typical of the Old World begomoviruses. Sequence identities among the three isolates ranged from 99.7 to 99.8%. When compared with other reported sequences of begomoviruses, GD13 was most closely related to papaya leaf curl China virus (AJ876548), with a sequence identity of 76.8%. In addition, all isolates were found to be associated with DNAβ molecules. The complete DNAβ sequences of isolates GD13, GD24 and GD27 were determined. Sequence analysis showed that they had highest sequence identity with DNAβ of Eupatorium yellow vein virus (AJ438938) (44.0, 43.9 and 45.6% identity). GD13, GD24 and GD27 are considered to be isolates of a distinct begomovirus species for which the name Siegesbeckia yellow vein virus (SgYVV) is proposed.
Archives of Virology – Springer Journals
Published: Apr 1, 2007
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