Sialic acid content in human saliva and anti-influenza activity against human and avian influenza viruses

Sialic acid content in human saliva and anti-influenza activity against human and avian influenza... It was shown previously that human saliva has higher antiviral activity against human influenza viruses than against H5N1 highly pathogenic avian influenza viruses, and that the major anti-influenza activity was associated with sialic-acid-containing molecules. To further characterize the differential susceptibility to saliva among influenza viruses, seasonal influenza A and B virus, pandemic H1N1 virus, and 15 subtypes of avian influenza virus were tested for their susceptibility to human and chicken saliva. Human saliva showed higher hemagglutination inhibition (HI) and neutralization (NT) titers against seasonal influenza A virus and the pandemic H1N1 viruses than against influenza B virus and most avian influenza viruses, except for H9N2 and H12N9 avian influenza viruses, which showed high HI and NT titers. To understand the nature of sialic-acid-containing anti-influenza factors in human saliva, α2,3- and α2,6-linked sialic acid was measured in human saliva samples using a lectin binding and dot blot assay. α2,6-linked sialic acid was found to be more abundant than α2,3-linked sialic acid, and a seasonal H1N1 influenza virus bound more efficiently to human saliva than an H5N1 virus in a dot blot analysis. These data indicated that human saliva contains the sialic acid type corresponding to the binding preference of seasonal influenza viruses. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Sialic acid content in human saliva and anti-influenza activity against human and avian influenza viruses

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Publisher
Springer Vienna
Copyright
Copyright © 2016 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-015-2700-z
Publisher site
See Article on Publisher Site

Abstract

It was shown previously that human saliva has higher antiviral activity against human influenza viruses than against H5N1 highly pathogenic avian influenza viruses, and that the major anti-influenza activity was associated with sialic-acid-containing molecules. To further characterize the differential susceptibility to saliva among influenza viruses, seasonal influenza A and B virus, pandemic H1N1 virus, and 15 subtypes of avian influenza virus were tested for their susceptibility to human and chicken saliva. Human saliva showed higher hemagglutination inhibition (HI) and neutralization (NT) titers against seasonal influenza A virus and the pandemic H1N1 viruses than against influenza B virus and most avian influenza viruses, except for H9N2 and H12N9 avian influenza viruses, which showed high HI and NT titers. To understand the nature of sialic-acid-containing anti-influenza factors in human saliva, α2,3- and α2,6-linked sialic acid was measured in human saliva samples using a lectin binding and dot blot assay. α2,6-linked sialic acid was found to be more abundant than α2,3-linked sialic acid, and a seasonal H1N1 influenza virus bound more efficiently to human saliva than an H5N1 virus in a dot blot analysis. These data indicated that human saliva contains the sialic acid type corresponding to the binding preference of seasonal influenza viruses.

Journal

Archives of VirologySpringer Journals

Published: Mar 1, 2016

References

  • Assessment of the antiviral properties of recombinant surfactant protein D against influenza B virus in vitro
    Hillaire, ML; Eijk, M; Vogelzang-van Trierum, SE; Nieuwkoop, NJ; Riel, D; Fouchier, RA; Kuiken, T; Osterhaus, AD; Haagsman, HP; Rimmelzwaan, GF
  • Susceptibility of human and avian influenza viruses to human and chicken saliva
    Limsuwat, N; Suptawiwat, O; Boonarkart, C; Puthavathana, P; Auewarakul, P; Wiriyarat, W
  • Respiratory tract mucin genes and mucin glycoproteins in health and disease
    Rose, MC; Voynow, JA
  • Altered sialyl- and fucosyl-linkage on mucins in cystic fibrosis patients promotes formation of the sialyl-Lewis X determinant on salivary MUC-5B and MUC-7
    Shori, DK; Genter, T; Hansen, J; Koch, C; Wyatt, H; Kariyawasam, HH; Knight, RA; Hodson, ME; Kalogeridis, A; Tsanakas, I

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