Shoot regeneration from cotyledon explants of fibre flax (Linum usitatissimum) and their subsequent rooting

Shoot regeneration from cotyledon explants of fibre flax (Linum usitatissimum) and their... In this study, the morphogenic ability of cotyledon explants of fibre flax (Linum usitatissimum L.) was investigated in relation to their physiological age and genotype and the effect of growth regulators in the medium. Among four investigated genotypes, the explants of cvs. A-29 and Alexim manifested the highest and the lowest morphogenic ability, respectively. The optimum ratio of growth regulators for shoot regeneration was shown to depend on the age of donor plantlets from which the explants were obtained. For explants excised from younger plantlets, the lower concentration of benzyladenine (BA) and the absence of auxin were preferable. For explants from older plantlets, the higher BA concentration and NAA presence in the medium were required for shoot regeneration. The addition of ABA to the regeneration medium usually reduced shoot regeneration frequency; however, the inhibitory effect of ABA depended on other growth regulators. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Shoot regeneration from cotyledon explants of fibre flax (Linum usitatissimum) and their subsequent rooting

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Publisher
Nauka/Interperiodica
Copyright
Copyright © 2006 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S102144370604011X
Publisher site
See Article on Publisher Site

Abstract

In this study, the morphogenic ability of cotyledon explants of fibre flax (Linum usitatissimum L.) was investigated in relation to their physiological age and genotype and the effect of growth regulators in the medium. Among four investigated genotypes, the explants of cvs. A-29 and Alexim manifested the highest and the lowest morphogenic ability, respectively. The optimum ratio of growth regulators for shoot regeneration was shown to depend on the age of donor plantlets from which the explants were obtained. For explants excised from younger plantlets, the lower concentration of benzyladenine (BA) and the absence of auxin were preferable. For explants from older plantlets, the higher BA concentration and NAA presence in the medium were required for shoot regeneration. The addition of ABA to the regeneration medium usually reduced shoot regeneration frequency; however, the inhibitory effect of ABA depended on other growth regulators.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Jul 7, 2006

References

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