Both the inactive and active conformations of the hemolysin/cytolysin of Serratia marcescens (ShlA) binds membranes of erythrocytes, but only active ShlA is able to form pores. ShlA is unable to lyse prokaryotic membranes. To determine the receptors of the binding and pore-forming domains of active cytolysin on eukaryotic membranes, artificial large unilamellar vesicles (LUVs) of various membrane compositions were examined. In the current study, it is shown that significant pore formation and lysis was achieved with binary phosphatidylcholine/phosphatidylserine (PS) liposomes. No proteinaceous receptor was needed for either binding or pore formation by ShlA. Membrane integration and pore-forming activity were enhanced by addition of phosphatidylethanolamine. Phosphatidylserine is negatively charged at physiologic pH and is almost absent in prokaryotic membranes. Hence, membrane binding and insertion of ShlA are highly dependent on phosphatidylserine, which targets the toxic activity to eukaryotic cell membranes without any need of a proteinaceous receptor. This may explain why prokaryotic membranes were found to be resistant against ShlA in a previous study.
The Journal of Membrane Biology – Springer Journals
Published: Sep 1, 2002
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera