Serological diagnosis of Mycoplasma pneumoniae infection by using the mimic epitopes

Serological diagnosis of Mycoplasma pneumoniae infection by using the mimic epitopes Nowadays, there is lack of effective serological detection method for Mycoplasma pneumoniae (M. pneumoniae) infection in clinic. In this study, the mimic epitopes of M. pneumoniae were screened to evaluate the role in the serodiagnosis of M. pneumoniae infection. The M. pneumoniae-positive serum was used as the target for biopanning to phage display random 7-peptide library. The positive phage clones were selected and the DNA were sequenced and analyzed by BLAST. The representative phages were identified using dot immunoblotting and ELISA. The exogenous heptapeptides were synthesized and their reactions with M. pneumonia-positive serum were tested by indirect ELISA. Two heptapeptides, namely heptapeptide 1: TVNFKLY and heptapeptide 2: LPQRLRT, were screened out from the randomly selected 40 phages after the four bio-panning rounds. They had high homologies to some M. pneumoniae antigens. Besides, the representative bacteriophage containing heptapeptide 1 or 2 could react with the M. pneumonia- positive serum. The sensitivities of heptapeptide 1 and heptapeptide 2 for the diagnosis of M. pneumoniae infection were 90.1 and 80.0%, respectively, and the specificities were 94.3 and 97.1%, respectively. Therefore the two heptapeptides were the mimic epitopes of M. pneumoniae and might have potential serological diagnosis value for M. pneumoniae infection. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png World Journal of Microbiology and Biotechnology Springer Journals

Serological diagnosis of Mycoplasma pneumoniae infection by using the mimic epitopes

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Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer Science+Business Media B.V., part of Springer Nature
Subject
Life Sciences; Microbiology; Biotechnology; Biochemistry, general; Environmental Engineering/Biotechnology; Applied Microbiology
ISSN
0959-3993
eISSN
1573-0972
DOI
10.1007/s11274-018-2467-y
pmid
29845357
Publisher site
See Article on Publisher Site

Abstract

Nowadays, there is lack of effective serological detection method for Mycoplasma pneumoniae (M. pneumoniae) infection in clinic. In this study, the mimic epitopes of M. pneumoniae were screened to evaluate the role in the serodiagnosis of M. pneumoniae infection. The M. pneumoniae-positive serum was used as the target for biopanning to phage display random 7-peptide library. The positive phage clones were selected and the DNA were sequenced and analyzed by BLAST. The representative phages were identified using dot immunoblotting and ELISA. The exogenous heptapeptides were synthesized and their reactions with M. pneumonia-positive serum were tested by indirect ELISA. Two heptapeptides, namely heptapeptide 1: TVNFKLY and heptapeptide 2: LPQRLRT, were screened out from the randomly selected 40 phages after the four bio-panning rounds. They had high homologies to some M. pneumoniae antigens. Besides, the representative bacteriophage containing heptapeptide 1 or 2 could react with the M. pneumonia- positive serum. The sensitivities of heptapeptide 1 and heptapeptide 2 for the diagnosis of M. pneumoniae infection were 90.1 and 80.0%, respectively, and the specificities were 94.3 and 97.1%, respectively. Therefore the two heptapeptides were the mimic epitopes of M. pneumoniae and might have potential serological diagnosis value for M. pneumoniae infection.

Journal

World Journal of Microbiology and Biotechnology – Springer Journals

Published: May 29, 2018

References

  • Processing is required for a fully functional protein P30 in Mycoplasma pneumoniae gliding and cytadherence
    Chang, HY; Prince, OA; Sheppard, ES; Krause, DC
  • Application of phage peptide display technology for the study of food allergen epitopes
    Chen, X; Dreskin, SC
  • Delineation of immunodominant and cytadherence segment(s) of Mycoplasma pneumoniae P1 gene
    Chourasia, BK; Chaudhry, R; Malhotra, P
  • Subtractive phage display selection from canine visceral leishmaniasis identifies novel epitopes that mimic Leishmania infantum antigens with potential serodiagnosis applications
    Costa, LE
  • Mimotope identification of dust mite allergen Der f 5 using phagedisplayed random peptide libraries
    Cui, Y; Yu, L; Zhou, Y; Yang, L; Zhang, C
  • The evolution of advanced molecular diagnostics for the detection and characterization of Mycoplasma pneumoniae
    Diaz, MH; Winchell, JM
  • Value of serum Mycoplasma pneumoniae immunoglobulin in the diagnosis of mycoplasma-related pneumonia in newborns
    Dong, Y; Lv, W; Lin, Z
  • Screening hybridomas for anabolic androgenic steroids by steroid analog antigen microarray
    Du, H
  • Comparison of serological methods with PCR-based methods for the diagnosis of community-acquired pneumonia caused by atypical bacteria
    Herrera, M; Aguilar, YA; Rueda, ZV; Muskus, C; Velez, LA
  • A library of plasmodium vivax recombinant merozoite proteins reveals new vaccine candidates and protein-protein interactions
    Hostetler, JB; Sharma, S; Bartholdson, SJ; Wright, GJ; Fairhurst, RM; Rayner, JC
  • Mycoplasma pneumoniae: a cross-sectional population-based comparison of disease severity in preschool and school-age children
    Inchley, CS; Berg, AS; Vahdani Benam, A; Kvissel, AK; Leegaard, TM; Nakstad, B
  • The alpha/beta hydrolase fold proteins of Mycobacterium tuberculosis, with reference to their contribution to virulence
    Johnson, G
  • Community-acquired pneumonia caused by Mycoplasma pneumoniae: how physical and radiological examination contribute to successful diagnosis
    Kishaba, T
  • Phage-displayed peptides that mimic epitopes of hepatitis E virus capsid
    Larralde, O; Petrik, J
  • Computer-predicted peptides that mimic discontinuous epitopes on the A2 domain of factor VIII
    Lebreton, A
  • Early serologic diagnosis of Mycoplasma pneumoniae pneumonia: an observational study on changes in titers of specific-IgM antibodies and cold agglutinins
    Lee, SC; Youn, YS; Rhim, JW; Kang, JH; Lee, KY
  • Role of serum Mycoplasma pneumoniae IgA, IgM, and IgG in the diagnosis of Mycoplasma pneumoniae-related Pneumonia in school-age children and adolescents
    Lee, WJ
  • Rapid diagnosis of Mycoplasma pneumoniae in children with pneumonia by an immuno-chromatographic antigen assay
    Li, W; Liu, Y; Zhao, Y; Tao, R; Li, Y; Shang, S
  • Evaluation of a real-time method of simultaneous amplification and testing in diagnosis of Mycoplasma pneumoniae infection in children with pneumonia
    Li, W; Fang, YH; Shen, HQ; Yang, DH; Shu, Q; Shang, SQ
  • B cell cross-epitope of Propionibacterium acnes and Actinobacillus pleuropneumonia selected by phage display library can efficiently protect from Actinobacillus pleuropneumonia infection
    Liu, J
  • Computational prediction of immunodominant antigenic regions & potential protective epitopes for dengue vaccination
    Muthusamy, K; Gopinath, K; Nandhini, D
  • Virally vectored vaccine delivery: medical needs, mechanisms, advantages and challenges
    Pinschewer, DD
  • Functional epitope core motif of the Anaplasma marginale major surface protein 1a and its incorporation onto bioelectrodes for antibody detection
    Santos, PS
  • Role of an N-terminal extension in stability and catalytic activity of a hyperthermostable alpha/beta hydrolase fold esterase
    Singh, MK; Shivakumaraswamy, S; Gummadi, SN; Manoj, N
  • Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface
    Smith, GP
  • Vaccination with phage-displayed antigenic epitope
    Wang, Y; Wang, L
  • The mimic epitopes of Mycobacterium tuberculosis screened by phage display peptide library have serodiagnostic potential for tuberculosis
    Wang, L
  • A comparison study between GeXP-based multiplex-PCR and serology assay for Mycoplasma pneumoniae detection in children with community acquired pneumonia
    Wang, L
  • P40 and P90 from Mpn142 are targets of multiple processing events on the surface of Mycoplasma pneumoniae
    Widjaja, M; Berry, IJ; Pont, EJ; Padula, MP; Djordjevic, SP
  • Identification of three novel B-cell epitopes of VMH protein from Vibrio mimicus by screening a phage display peptide library
    Xiao, N; Cao, J; Zhou, H; Ding, SQ; Kong, LY; Li, JN
  • Evaluation of P1 adhesin epitopes for the serodiagnosis of Mycoplasma pneumoniae infections
    Xue, G; Cao, L; Wang, L; Zhao, H; Feng, Y; Ma, L; Sun, H
  • Identification of rabies virus mimotopes screened from a phage display peptide library with purified dog anti-rabies virus serum IgG
    Yang, L; Cen, J; Xue, Q; Li, J; Bi, Y; Sun, L; Liu, W
  • Screening and identification of the mimic epitope of the adhesion protein of Mycoplasma genitalium
    Zeng, Y; Liu, L; He, J; Liu, Y; Zhu, C; You, X; Wu, Y
  • Translational quality control by bacterial threonyl-tRNA synthetases
    Zhou, XL

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