Sequencing and characterization of the coat protein and 3 ′ non-coding region of a newsweet potato potyvirus

Sequencing and characterization of the coat protein and 3 ′ non-coding region of a newsweet... A cDNA library was constructed from viral genomic RNA purified from sweet potato plants affected by “Sweet Potato Chlorotic Dwarf disease” in an attempt to clarify the etiology of this viral complex in Argentina. By sequence analysis, some of the obtained clones were found to belong to sweet potato feathery mottle potyvirus (SPFMV), to a closterovirus and to a new potyvirus. A cDNA clone of 1,103 bp representing the coat protein cistron and 3 ′ non-coding region of the newly identified potyvirus was further characterized. The sequence contained an ORF of 855 nucleotides with a coding capacity of 285 amino acids, followed by a 3 ′ untranslated tail of 248 nucleotides. The core and C-terminal regions have sequences well conserved among potyviruses. Furthermore, amino acid sequence comparisons of the capsid protein with those of other described potyviruses showed 63% homology with SPFMV, 68 to 70% with two different isolates of sweet potato latent potyvirus (SPLV), 57% with sweet potato G potyvirus (SPGV) and 73% with potato virus Y (PVY). These data allowed us to propose the inclusion of this virus as a new member of the family Potyviridae , genus Potyvirus with the designation sweet potato mild speckling potyvirus (SPMSV). Archives of Virology Springer Journals

Sequencing and characterization of the coat protein and 3 ′ non-coding region of a newsweet potato potyvirus

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Copyright © 1997 by Springer-Verlag/Wien
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