Arch Virol (2006) 151: 827–835
Sequence variability of the human cytomegalovirus
UL141 Open Reading Frame in clinical strains
Y.-P. Ma, Q. Ruan, R. He, Y. Qi, Z.-R. Sun, Y.-H. Ji, Y.-J. Huang, Q. Liu,
S.-R. Chen, and J.-D. Wang
Virus Laboratory, 2
Afﬁliated Hospital, China Medical University,
Shenyang, P.R. China
Received July 11, 2005; accepted August 15, 2005
Published online September 30, 2005
Summary. Human cytomegalovirus (HCMV) displays genetic polymorphisms.
HCMV disease and tissue tropism may be related to speciﬁc genomic variability
among strains. This work analyzed the genetic polymorphism of UL141 open
reading frame (ORF), one of the genes in HCMV UL/b
region, from 21 clinical
strains. 8 previously published UL141 sequences in the GenBank were used for
sequence comparison. Detailed sequence analysis showed that the UL141 gene
was highly conserved at both the nucleotide and amino acid level. The coding
regions were identical in size. The nucleotide and amino acid sequence identities
among all strains were 96.9–100% and 97.6–100%, respectively.
Human cytomegalovirus (HCMV), a member of the family Herpesviridae, sub-
family Betaherpesvirinae, genus Cytomegalovirus, is a ubiquitous β-herpesvirus
and the leading cause of congenital viral infection. HCMV infection can be trans-
mitted during pregnancy from the mother with primary and recurrent infection to
the fetus. Primary infection in pregnant women can lead to severe disease to fetus
and infants, including microcephaly, intracranial calciﬁcation, hydrocephalus,
hepatosplenomegaly, severe liver dysfunction, congenital megacolon. [1, 7, 15, 19]
The diversity of organs and cell types infected by HCMV in vivo has led to the
Supported by the National Natural Science Foundation of China (30170986).
Note: Nucleotide sequence data reported are available in the GenBank databases under the
accession numbers AY496547–AY496555, AY600459–AY600468, AY941104–AY941105.