Mammalian Genome 9, 775–777 (1998). Incorporating Mouse Genome © Springer-Verlag New York Inc. 1998 Sequence of a 42-kb mouse region containing the imprinted H19 locus: identification of a novel muscle-specific transcription unit showing biallelic expression Kou Ishihara, Reike Kato, Hiroyasu Furuumi, Mohamad Zubair, Hiroyuki Sasaki Division of Disease Genes, Institute of Genetic Information, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan Received: 26 March 1998 / Accepted: 26 May 1998 The mouse H19 gene, encoding an untranslated RNA with un- 27495–27795 of our sequence with four mismatches (Fig. 2A). known function, is subject to genomic imprinting and expressed The gap between the two regions started with GT, ended with AG, exclusively from the maternal allele (Bartolomei et al. 1991). The and contained other features of an intron. We then examined RNA gene is located in the distal region of Chromosome (Chr) 7 within samples from various mouse organs by Northern blotting, using a G-bands F4/F5, which contains at least five other imprinted genes genomic fragment containing the 38 part of the EST as a probe. (Beechey et al. 1997). Among these, the insulin-like growth factor Two different RNA species, 2.7 and 1.0 kb in size, were detected II (Igf2)
Mammalian Genome – Springer Journals
Published: Sep 1, 1998
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