In order to determine the diversity of the movement protein (NS M ) among tospoviruses, the NS M genes of five distinct tospovirus species occurring in Brazil ( Tomato chlorotic spot virus , Groundnut ring spot virus , Chrysanthemum stem necrosis virus , Zucchini lethal chlorosis virus and Iris yellow spot virus ) were cloned, sequenced and compared with NS M sequences of other available tospoviruses. The ‘D-motif’, a conserved region present in the majority of ‘30K superfamily’ virus movement proteins, is present in all NS M amino acid sequences available. In addition to the ‘D-motif’, a conserved phospholipase A2 motif was found. The NS M amino acid sequence comparisons among tospovirus species revealed several conserved regions located in the internal part of the protein and diverse domains mainly located in the amino-terminus. Prediction of secondary structure showed similar patterns among all NS M proteins analyzed. Considering the geographical prevalence and phylogenetic analysis of N and NS M proteins, tospoviruses were tentatively clustered in ‘American’ and ‘Eurasian’ groups. Both phylogenetic trees may reflect the natural evolution of tospovirus species within distinct ecological niches. The sequence information obtained in this work would facilitate functional analysis of NS M during the tospovirus infection process.
Archives of Virology – Springer Journals
Published: Jul 1, 2001
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera