Sequence announcements

Sequence announcements Plant Molecular Biology 38: 911–912, 1998. © 1998 Kluwer Academic Publishers. Printed in the Netherlands. GenBank accession #: AF036580 Authors: Nelson AJ, Apel-Birkhold PC, Bailey BA Address: Biocontrol of Plant Diseases Laboratory, Bldg. 011A, Rm. 275, BARC-West, ARS, USDA, Beltsville, MD 20705, USA Source of sequence: Partial amino acid sequences were obtained for a purified 24 kDa protein from Fusarium oxysporum f. sp. erythroxyli that induces necrosis and ethylene production in leaves of Erythroxylum coca and other dicots. A DNA fragment was amplified from genomic DNA prepared from 3-day-old mycelial cultures using degenerate primers (9501, 9506) based on the partial amino acid sequences, and a full length genomic clone was isolated from a DASH II genomic library using the amplified DNA fragment as a probe. Trivial name: Nep1, encodes necrosis and ethylene inducing peptide (NEP1) from Fusarium oxysporum f. sp. erythroxyli. Description: The coding sequence consists of two exons interrupted by a single intron of 58 nucleotides, and encodes a protein of 253 amino acids. A TATA box occurs at137 to132, multiple transcription start sites at 107 to 93, and a putative polyadenylation signal at C970 to C975. The encoded protein is mostly basic and hydrophylic, and contains a Plant Molecular Biology Springer Journals

Sequence announcements

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Kluwer Academic Publishers
Copyright © 1998 by Kluwer Academic Publishers
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
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