Sequence and comparative structural analysis of the murine leukaemia virus amphotropic strain 4070A RNase H domain

Sequence and comparative structural analysis of the murine leukaemia virus amphotropic strain... The sequence of a 900-nucleotide segment (encoding part of the reverse transcriptase, including the entire RNase H domain) of the pol gene of the murine leukaemia virus (MLV) amphotropic strain 4070A is presented. Alignment of the inferred 4070A RNase H amino acid sequence (157 residues) with other MLV RNase H sequences revealed only minor differences compared with the divergence between other retroviral and prokaryotic or eukaryotic RNase H sequences. Only 10 residues were invariant across the entire sample set, but secondary structure predictions for the enzymes from E. coli , yeast, human liver and diverse retroviruses (HIV, Rous sarcoma virus, foamy viruses) supported, in every case, the five β-strands (1 to 5) and four or five α-helices (A, B/C, D, E) that have been identified by crystallography in the RNase H domain of HIV-1 reverse transcriptase and in E. coli RNase H. In the case of MLV, analysis of the RNase H domain sequences inferred from 10 different strains (including the amphotropic 4070A) predicted all five α-helices (A–E), as well as β-strands 4 and 5. However, the N-terminal segment (residues 1–40) was predicted, without exception and with high probability, to fold uniquely into one (or two adjacent) α-helix(es) encompassing residues 13–37, instead of the three β-strands known to exist in the HIV-1 and E. coli enzymes. The unerring consistency between the known and predicted structures of the HIV-1 and E. coli enzymes, and the prediction of the same structural elements (including β-strands 1–3 within the N-terminal segment) for all other (non-MLV) RNase H proteins examined in this study, suggests that the N-terminal segment of the MLV RNase H domain assumes a conformation distinct from that of other retroviral and cellular RNase H molecules. An additional (sixth) β-strand was also predicted uniquely within the C-terminal region of foamy virus RNase H domains. Archives of Virology Springer Journals

Sequence and comparative structural analysis of the murine leukaemia virus amphotropic strain 4070A RNase H domain

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