Sequence analysis of the replicase gene of ‘sweet potato caulimo-like virus’ suggests that this virus is a distinct member of the genus Cavemovirus

Sequence analysis of the replicase gene of ‘sweet potato caulimo-like virus’ suggests that... Virion purification from indicator plants and partial sequencing of the replicase region of a ‘sweet potato caulimo-like virus’ (SPCV) isolate from Madeira, Portugal, are described. Phylogenetic analysis suggests that SPCV is a distinct member of the genus Cavemovirus (family Caulimoviridae ). These results explain previous failed attempts to characterize SPCV based on antibodies or primers designed for other members of the Caulimoviridae . Using a quick DNA extraction protocol and PCR primers flanking the RT motif region, we were able to detect SPCV directly in sweet potato, thus saving considerable time during routine virus indexing. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Sequence analysis of the replicase gene of ‘sweet potato caulimo-like virus’ suggests that this virus is a distinct member of the genus Cavemovirus

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Publisher
Springer Journals
Copyright
Copyright © 2011 by Springer-Verlag
Subject
Biomedicine; Virology ; Medical Microbiology ; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-010-0886-7
Publisher site
See Article on Publisher Site

Abstract

Virion purification from indicator plants and partial sequencing of the replicase region of a ‘sweet potato caulimo-like virus’ (SPCV) isolate from Madeira, Portugal, are described. Phylogenetic analysis suggests that SPCV is a distinct member of the genus Cavemovirus (family Caulimoviridae ). These results explain previous failed attempts to characterize SPCV based on antibodies or primers designed for other members of the Caulimoviridae . Using a quick DNA extraction protocol and PCR primers flanking the RT motif region, we were able to detect SPCV directly in sweet potato, thus saving considerable time during routine virus indexing.

Journal

Archives of VirologySpringer Journals

Published: Mar 1, 2011

References

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