Sequence analysis of double-strand RNA6 and RNA9 from the fungus Sclerotium hydrophilum

Sequence analysis of double-strand RNA6 and RNA9 from the fungus Sclerotium hydrophilum We have recently reported the identification of 10 double-strand RNA segments from Sclerotium hydrophilum [HZ11] mycelia and of virus-like particles isolated from the mycelia, as well as the sequences of dsRNA2 and dsRNA7. Phylogenetic analysis revealed that dsRNA2 and dsRNA7 belong to a group of unclassified viruses. In this report, we cloned and sequenced dsRNA6 and dsRNA9 from the 10 dsRNAs. We tentatively named the putative virus “Sclerotium hydrophilum virus 1”, with isolates being abbreviated to ShV1, with dsRNA6 and dsRNA9 corresponding to dsRNA1 and dsRNA2, respectively, of ShV1. dsRNA1 was 1975 bp in length and encoded a putative RNA-dependent RNA polymerase (RdRp). dsRNA2 was 1728 bp and encoded a putative coat protein (CP). Phylogenetic analysis showed that the proteins encoded by dsRNA1 and dsRNA2 were highly related to known viral RdRps and CP, respectively, of viruses classified within the genus Alphapartitivirus of the family Partitiviridae. These members include Rhizoctonia solani dsRNA virus 2, Diuris pendunculata cryptic virus, and Heterobasidion partitivirus. The 5′- and 3′-untranslated regions (UTRs) of the two dsRNAs showed a high sequence identity. The 5′-UTR contained conserved sequences 5′-GAAGCAUCACUU(/G) G(/U)AGU(/A)UCGC(/U)CCA(/G) CAAUAACGAA-3′ and 5′-AAAUUGAUCUUACCUCUCAC-3′. The 3′-UTR contained the conserved sequence 5′-UUGUUUU-3′ and 5′-UUUA(/U)A(/C) UUAU-3′. These results indicate that dsRNA1 and dsRNA2 are phylogenetically related to members of the genus Alphapartitivirus of family Partitiviridae. We therefore propose that dsRNA1 and dsRNA2 are the genome sequences of a new partitivirus, ShV1. Archives of Virology Springer Journals

Sequence analysis of double-strand RNA6 and RNA9 from the fungus Sclerotium hydrophilum

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Springer Vienna
Copyright © 2017 by Springer-Verlag Wien
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
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