Separation of Piper kadsura Using Preparative Supercritical Fluid Chromatography Combined with Preparative Reversed-Phase Liquid Chromatography

Separation of Piper kadsura Using Preparative Supercritical Fluid Chromatography Combined with... We have developed a separation strategy that employed preparative supercritical fluid chromatography (prep-SFC) com- bined with preparative reversed-phase liquid chromatography (prep-RPLC) to purify compounds from Piper kadsura. The complexity of the sample was effectively reduced by two steps of prep-SFC. The key experimental parameters of prep-SFC were systematically optimized, including an “average-pressure rule” scaling-up method for adjustment of the backpressure of the system. A mixed modifier consisting of ethanol/ n-hexane was applied to provide a stable elution. Prep-RPLC was subsequently adopted to purify the SFC fractions due to its good orthogonality with SFC. Furthermore, chiral SFC was used to separate the structural analogues. As a result, 14 compounds with high purity were obtained from one fraction, including three pairs of diastereoisomers. These data indicate that the separation strategy using prep-SFC combined with prep-RPLC is an effective tool to separate complex samples such as natural products. Keywords Supercritical fluid chromatography · Reversed-phase liquid chromatography · Purification · Natural products · Piper kadsura Introduction for each compound make full separation difficult. Thus, two key problems are involved in a separation task: how to sim- Separation of compounds from natural products remains a plify the sample and how to improve the separation selec- challenge, which is http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Chromatographia Springer Journals

Separation of Piper kadsura Using Preparative Supercritical Fluid Chromatography Combined with Preparative Reversed-Phase Liquid Chromatography

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Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2018 by Springer-Verlag GmbH Germany, part of Springer Nature
Subject
Chemistry; Chromatography; Proteomics; Pharmacy; Laboratory Medicine; Analytical Chemistry
ISSN
0009-5893
eISSN
1612-1112
D.O.I.
10.1007/s10337-018-3544-y
Publisher site
See Article on Publisher Site

Abstract

We have developed a separation strategy that employed preparative supercritical fluid chromatography (prep-SFC) com- bined with preparative reversed-phase liquid chromatography (prep-RPLC) to purify compounds from Piper kadsura. The complexity of the sample was effectively reduced by two steps of prep-SFC. The key experimental parameters of prep-SFC were systematically optimized, including an “average-pressure rule” scaling-up method for adjustment of the backpressure of the system. A mixed modifier consisting of ethanol/ n-hexane was applied to provide a stable elution. Prep-RPLC was subsequently adopted to purify the SFC fractions due to its good orthogonality with SFC. Furthermore, chiral SFC was used to separate the structural analogues. As a result, 14 compounds with high purity were obtained from one fraction, including three pairs of diastereoisomers. These data indicate that the separation strategy using prep-SFC combined with prep-RPLC is an effective tool to separate complex samples such as natural products. Keywords Supercritical fluid chromatography · Reversed-phase liquid chromatography · Purification · Natural products · Piper kadsura Introduction for each compound make full separation difficult. Thus, two key problems are involved in a separation task: how to sim- Separation of compounds from natural products remains a plify the sample and how to improve the separation selec- challenge, which is

Journal

ChromatographiaSpringer Journals

Published: May 31, 2018

References

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