Separation of 1,4-Dihydropyridine Derivative and Its Oxidized Form

Separation of 1,4-Dihydropyridine Derivative and Its Oxidized Form Derivatives of 1,4-dihydropyridine (DHP) still play an important role in treatment of cardiovascular diseases. Typical degradation of the DHP ring is aromatization to pyridine ring which occurs both chemically and biochemically. It is, therefore, important to have a reliable and robust analytical method for separation of DHPs from their oxidized counterparts. Separation of closely-related substances possessing similar hydrophobicity, such as DHP and its oxidized form, can be challenging on conventional alkyl-bonded sorbents. In this study, an impact of reversed-phase (RP) liquid chromatography conditions on separation of the DHP/Ox pair has been investigated. Initially, a systematic study has been performed on 33 commercial RP columns with mobile phase acetonitrile/water for separation of foridone and its corresponding oxidized form. The retention and selectivity are discussed in view of the hydrophobic-subtraction model. Best separation was found replacing conventional C18 sorbents with ones containing an embedded polar group due to polar interactions. Similarly, application of cyano columns resulted in efficient separation of analytes. Organic modifier of mobile phase (methanol vs. acetonitrile) contributed significantly to separation of foridone from its oxidized counterpart. Separation of six chemically diverse DHPs from corresponding oxidized forms was studied on seven RP columns (traditional C18 sorbent, alkyl sorbent with polar embedded group, two different aromatic phases, pentafluorophenylpropyl sorbent and sorbent with straight chain perfluorohexyl ligand). Both acetonitrile and methanol were applied as organic modifier. It was found that application of alkyl sorbent with an embedded polar group (column Zorbax Bonus RP) or cyano sorbent (column ACE CN) yields clear separation of chemically diverse DHPs from their oxidized forms. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Chromatographia Springer Journals

Separation of 1,4-Dihydropyridine Derivative and Its Oxidized Form

Loading next page...
 
/lp/springer_journal/separation-of-1-4-dihydropyridine-derivative-and-its-oxidized-form-WM5TRxh9Ek
Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer-Verlag GmbH Germany, part of Springer Nature
Subject
Chemistry; Chromatography; Proteomics; Pharmacy; Laboratory Medicine; Analytical Chemistry
ISSN
0009-5893
eISSN
1612-1112
D.O.I.
10.1007/s10337-018-3529-x
Publisher site
See Article on Publisher Site

Abstract

Derivatives of 1,4-dihydropyridine (DHP) still play an important role in treatment of cardiovascular diseases. Typical degradation of the DHP ring is aromatization to pyridine ring which occurs both chemically and biochemically. It is, therefore, important to have a reliable and robust analytical method for separation of DHPs from their oxidized counterparts. Separation of closely-related substances possessing similar hydrophobicity, such as DHP and its oxidized form, can be challenging on conventional alkyl-bonded sorbents. In this study, an impact of reversed-phase (RP) liquid chromatography conditions on separation of the DHP/Ox pair has been investigated. Initially, a systematic study has been performed on 33 commercial RP columns with mobile phase acetonitrile/water for separation of foridone and its corresponding oxidized form. The retention and selectivity are discussed in view of the hydrophobic-subtraction model. Best separation was found replacing conventional C18 sorbents with ones containing an embedded polar group due to polar interactions. Similarly, application of cyano columns resulted in efficient separation of analytes. Organic modifier of mobile phase (methanol vs. acetonitrile) contributed significantly to separation of foridone from its oxidized counterpart. Separation of six chemically diverse DHPs from corresponding oxidized forms was studied on seven RP columns (traditional C18 sorbent, alkyl sorbent with polar embedded group, two different aromatic phases, pentafluorophenylpropyl sorbent and sorbent with straight chain perfluorohexyl ligand). Both acetonitrile and methanol were applied as organic modifier. It was found that application of alkyl sorbent with an embedded polar group (column Zorbax Bonus RP) or cyano sorbent (column ACE CN) yields clear separation of chemically diverse DHPs from their oxidized forms.

Journal

ChromatographiaSpringer Journals

Published: May 31, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off