The sensitivity of calluses derived from susceptible and resistant goosegrass (Eleusine indica (L.) Gaertn.) biotypes to dinitroaniline herbicides, which disrupt interphase and mitotic-spindle microtubules, was evaluated. A callus culture derived from the resistant biotype retained resistance to both trifluralin (dinitroaniline herbicide) and amiprophosmethyl (phosphorothioamidate herbicide). The site for the interaction between α-tubulin subunit and dinitroaniline or phosphorothioamidate herbicides was identified by computer simulation. A correlation was found between the level of callus sensitivity to herbicide tested and the pattern of herbicide interaction with α-tubulin.
Russian Journal of Plant Physiology – Springer Journals
Published: Oct 13, 2004
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