The upstream sequence of pinb previously isolated from rice and confirmed to be a wound-inducible promoter by detecting GUS in T0 transgenic rice transformed via Agrobacterium tumefaciens-mediated procedures. In a transgenic line (pinb-16), the selectable marker hptII driven by CaMV35S promoter was completely silenced in T2 sublines; but the uidA gene driven by pinb promoter was expressed without being affected, though it, together with hptII, exists in the same T-DNA insertion. Analyses of methylation patterns using bisulphite-sequencing in the homozygous T1 and T2 sublines showed that cytosines in CaMV35S were gradually methylated in T1 plants and almost completely methylated in T2 plants. Interestingly, the process of methylation was accompanied by the occurrence of lesion mimic phenotype in rice leaves. The activity of hygromycin-resistance could be reestablished by treatment with 5-azacytidine. Genomic Southern and isolation of the T-DNA flanking sequences indicated that T-DNA was inserted in a retroelement of rice. These results revealed that methylation shows preference for the heterogeneity promoter fragment in the transgenic rice line and may be induced by the retroelement.
Russian Journal of Plant Physiology – Springer Journals
Published: Mar 24, 2006
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera