Selective methylation of one of the two promoters residing in T-DNA inserted in a retroelement of rice

Selective methylation of one of the two promoters residing in T-DNA inserted in a retroelement of... The upstream sequence of pinb previously isolated from rice and confirmed to be a wound-inducible promoter by detecting GUS in T0 transgenic rice transformed via Agrobacterium tumefaciens-mediated procedures. In a transgenic line (pinb-16), the selectable marker hptII driven by CaMV35S promoter was completely silenced in T2 sublines; but the uidA gene driven by pinb promoter was expressed without being affected, though it, together with hptII, exists in the same T-DNA insertion. Analyses of methylation patterns using bisulphite-sequencing in the homozygous T1 and T2 sublines showed that cytosines in CaMV35S were gradually methylated in T1 plants and almost completely methylated in T2 plants. Interestingly, the process of methylation was accompanied by the occurrence of lesion mimic phenotype in rice leaves. The activity of hygromycin-resistance could be reestablished by treatment with 5-azacytidine. Genomic Southern and isolation of the T-DNA flanking sequences indicated that T-DNA was inserted in a retroelement of rice. These results revealed that methylation shows preference for the heterogeneity promoter fragment in the transgenic rice line and may be induced by the retroelement. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Selective methylation of one of the two promoters residing in T-DNA inserted in a retroelement of rice

Loading next page...
 
/lp/springer_journal/selective-methylation-of-one-of-the-two-promoters-residing-in-t-dna-3EPAAlfNW9
Publisher
Nauka/Interperiodica
Copyright
Copyright © 2006 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443706020142
Publisher site
See Article on Publisher Site

Abstract

The upstream sequence of pinb previously isolated from rice and confirmed to be a wound-inducible promoter by detecting GUS in T0 transgenic rice transformed via Agrobacterium tumefaciens-mediated procedures. In a transgenic line (pinb-16), the selectable marker hptII driven by CaMV35S promoter was completely silenced in T2 sublines; but the uidA gene driven by pinb promoter was expressed without being affected, though it, together with hptII, exists in the same T-DNA insertion. Analyses of methylation patterns using bisulphite-sequencing in the homozygous T1 and T2 sublines showed that cytosines in CaMV35S were gradually methylated in T1 plants and almost completely methylated in T2 plants. Interestingly, the process of methylation was accompanied by the occurrence of lesion mimic phenotype in rice leaves. The activity of hygromycin-resistance could be reestablished by treatment with 5-azacytidine. Genomic Southern and isolation of the T-DNA flanking sequences indicated that T-DNA was inserted in a retroelement of rice. These results revealed that methylation shows preference for the heterogeneity promoter fragment in the transgenic rice line and may be induced by the retroelement.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Mar 24, 2006

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from
Google Scholar,
PubMed
Create lists to
organize your research
Export lists, citations
Read DeepDyve articles
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off