Selection of a Tobacco Auxin-Habituated Cell Line and Its Growth Characteristics

Selection of a Tobacco Auxin-Habituated Cell Line and Its Growth Characteristics Culturing conditions and the dose of a chemical mutagen N-nitroso-N-methylurea were worked out for selecting auxin-independent (auxin-habituated) cell lines of tobacco (Nicotiana tabacum L.). A clone (strain B-4) that steadily grew on an auxin-free nutrient medium and did not express any traits of morphogenesis emerged at the frequency of 1 per 7 × 107 plated cells. Mutation was the cause of habituation in this cell line as corroborated by a low frequency of its emergence, the appearance of the habituation trait only after the mutagenic treatment, and the stability of habituation trait for many years when the cells were subcultured in both selective and nonselective media. The auxin-habituated strain B-4 grew more rapidly on an auxin-free medium, was cytokinin-dependent, resistant to high exogenous IAA concentrations, and incapable of morphogenesis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Selection of a Tobacco Auxin-Habituated Cell Line and Its Growth Characteristics

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Publisher
Springer Journals
Copyright
Copyright © 2003 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1023/A:1023878321827
Publisher site
See Article on Publisher Site

Abstract

Culturing conditions and the dose of a chemical mutagen N-nitroso-N-methylurea were worked out for selecting auxin-independent (auxin-habituated) cell lines of tobacco (Nicotiana tabacum L.). A clone (strain B-4) that steadily grew on an auxin-free nutrient medium and did not express any traits of morphogenesis emerged at the frequency of 1 per 7 × 107 plated cells. Mutation was the cause of habituation in this cell line as corroborated by a low frequency of its emergence, the appearance of the habituation trait only after the mutagenic treatment, and the stability of habituation trait for many years when the cells were subcultured in both selective and nonselective media. The auxin-habituated strain B-4 grew more rapidly on an auxin-free medium, was cytokinin-dependent, resistant to high exogenous IAA concentrations, and incapable of morphogenesis.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 17, 2004

References

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