Selectable marker independent transformation of recalcitrant maize inbred B73 and sorghum P898012 mediated by morphogenic regulators BABY BOOM and WUSCHEL2

Selectable marker independent transformation of recalcitrant maize inbred B73 and sorghum P898012... Plant Cell Rep (2017) 36:1477–1491 DOI 10.1007/s00299-017-2169-1 ORIGINAL ARTICLE Selectable marker independent transformation of recalcitrant maize inbred B73 and sorghum P898012 mediated by morphogenic regulators BABY BOOM and WUSCHEL2 1 2 2 • • • Muruganantham Mookkan Kimberly Nelson-Vasilchik Joel Hague 1 2 Zhanyuan J. Zhang Albert P. Kausch Received: 17 April 2017 / Accepted: 20 June 2017 / Published online: 5 July 2017 The Author(s) 2017. This article is an open access publication Abstract promoter with lox P sites which bracket the CRE, WUS, and Key message Discriminatory co-expression of maize BBM genes. A constitutive maize UBI promoter directs a BBM and WUS transcriptional factor genes promoted ZsGreen GFP expression cassette as a reporter outside of the somatic embryogenesis and efficient Agrobacterium- excision sites and provides transient, transgenic, and devel- mediated transformation of recalcitrant maize inbred opmental analysis. This was coupled with evidence for B73 and sorghum P898012 genotypes without use of a molecular integration and analysis of stable integration and selectable marker gene. desiccation inducible CRE-mediated excision. Agrobac- Abstract The use of morphogenic regulators to overcome terium-mediated transgenic introduction of this vector barriers in plant transformation is a revolutionary break- showed transient expression of GFP and induced http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell Reports Springer Journals

Selectable marker independent transformation of recalcitrant maize inbred B73 and sorghum P898012 mediated by morphogenic regulators BABY BOOM and WUSCHEL2

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Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2017 by The Author(s)
Subject
Life Sciences; Plant Sciences; Cell Biology; Biotechnology; Plant Biochemistry
ISSN
0721-7714
eISSN
1432-203X
D.O.I.
10.1007/s00299-017-2169-1
Publisher site
See Article on Publisher Site

Abstract

Plant Cell Rep (2017) 36:1477–1491 DOI 10.1007/s00299-017-2169-1 ORIGINAL ARTICLE Selectable marker independent transformation of recalcitrant maize inbred B73 and sorghum P898012 mediated by morphogenic regulators BABY BOOM and WUSCHEL2 1 2 2 • • • Muruganantham Mookkan Kimberly Nelson-Vasilchik Joel Hague 1 2 Zhanyuan J. Zhang Albert P. Kausch Received: 17 April 2017 / Accepted: 20 June 2017 / Published online: 5 July 2017 The Author(s) 2017. This article is an open access publication Abstract promoter with lox P sites which bracket the CRE, WUS, and Key message Discriminatory co-expression of maize BBM genes. A constitutive maize UBI promoter directs a BBM and WUS transcriptional factor genes promoted ZsGreen GFP expression cassette as a reporter outside of the somatic embryogenesis and efficient Agrobacterium- excision sites and provides transient, transgenic, and devel- mediated transformation of recalcitrant maize inbred opmental analysis. This was coupled with evidence for B73 and sorghum P898012 genotypes without use of a molecular integration and analysis of stable integration and selectable marker gene. desiccation inducible CRE-mediated excision. Agrobac- Abstract The use of morphogenic regulators to overcome terium-mediated transgenic introduction of this vector barriers in plant transformation is a revolutionary break- showed transient expression of GFP and induced

Journal

Plant Cell ReportsSpringer Journals

Published: Jul 5, 2017

References

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