Plant Cell Rep (2017) 36:1477–1491 DOI 10.1007/s00299-017-2169-1 ORIGINAL ARTICLE Selectable marker independent transformation of recalcitrant maize inbred B73 and sorghum P898012 mediated by morphogenic regulators BABY BOOM and WUSCHEL2 1 2 2 • • • Muruganantham Mookkan Kimberly Nelson-Vasilchik Joel Hague 1 2 Zhanyuan J. Zhang Albert P. Kausch Received: 17 April 2017 / Accepted: 20 June 2017 / Published online: 5 July 2017 The Author(s) 2017. This article is an open access publication Abstract promoter with lox P sites which bracket the CRE, WUS, and Key message Discriminatory co-expression of maize BBM genes. A constitutive maize UBI promoter directs a BBM and WUS transcriptional factor genes promoted ZsGreen GFP expression cassette as a reporter outside of the somatic embryogenesis and efﬁcient Agrobacterium- excision sites and provides transient, transgenic, and devel- mediated transformation of recalcitrant maize inbred opmental analysis. This was coupled with evidence for B73 and sorghum P898012 genotypes without use of a molecular integration and analysis of stable integration and selectable marker gene. desiccation inducible CRE-mediated excision. Agrobac- Abstract The use of morphogenic regulators to overcome terium-mediated transgenic introduction of this vector barriers in plant transformation is a revolutionary break- showed transient expression of GFP and induced
Plant Cell Reports – Springer Journals
Published: Jul 5, 2017
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