Selectable marker independent transformation of recalcitrant maize inbred B73 and sorghum P898012 mediated by morphogenic regulators BABY BOOM and WUSCHEL2

Selectable marker independent transformation of recalcitrant maize inbred B73 and sorghum P898012... Plant Cell Rep (2017) 36:1477–1491 DOI 10.1007/s00299-017-2169-1 ORIGINAL ARTICLE Selectable marker independent transformation of recalcitrant maize inbred B73 and sorghum P898012 mediated by morphogenic regulators BABY BOOM and WUSCHEL2 1 2 2 • • • Muruganantham Mookkan Kimberly Nelson-Vasilchik Joel Hague 1 2 Zhanyuan J. Zhang Albert P. Kausch Received: 17 April 2017 / Accepted: 20 June 2017 / Published online: 5 July 2017 The Author(s) 2017. This article is an open access publication Abstract promoter with lox P sites which bracket the CRE, WUS, and Key message Discriminatory co-expression of maize BBM genes. A constitutive maize UBI promoter directs a BBM and WUS transcriptional factor genes promoted ZsGreen GFP expression cassette as a reporter outside of the somatic embryogenesis and efficient Agrobacterium- excision sites and provides transient, transgenic, and devel- mediated transformation of recalcitrant maize inbred opmental analysis. This was coupled with evidence for B73 and sorghum P898012 genotypes without use of a molecular integration and analysis of stable integration and selectable marker gene. desiccation inducible CRE-mediated excision. Agrobac- Abstract The use of morphogenic regulators to overcome terium-mediated transgenic introduction of this vector barriers in plant transformation is a revolutionary break- showed transient expression of GFP and induced http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Cell Reports Springer Journals

Selectable marker independent transformation of recalcitrant maize inbred B73 and sorghum P898012 mediated by morphogenic regulators BABY BOOM and WUSCHEL2

Loading next page...
 
/lp/springer_journal/selectable-marker-independent-transformation-of-recalcitrant-maize-zmR9y0C33l
Publisher
Springer Berlin Heidelberg
Copyright
Copyright © 2017 by The Author(s)
Subject
Life Sciences; Plant Sciences; Cell Biology; Biotechnology; Plant Biochemistry
ISSN
0721-7714
eISSN
1432-203X
D.O.I.
10.1007/s00299-017-2169-1
Publisher site
See Article on Publisher Site

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial