Screening of Phenolic Antioxidants in Edible Oils by HPTLC-DPPH Assay and MS Confirmation

Screening of Phenolic Antioxidants in Edible Oils by HPTLC-DPPH Assay and MS Confirmation The cytotoxicity of phenolic antioxidants had attracted marked attention, posing serious challenges to food safety. This paper presented a screening method for two major phenolic antioxidants (butylated hydroxytoluene and tert-butylhydroquinone) added in edible oils. To specifically visualize the targeted compounds after developing with toluene/ethyl acetate/methanol 8:1:1 (v/v/v) to 70 mm solvent front, the plate was subjected to a standardized 1,1-diphenyl-2-picrylhydrazyl assay. In addition to synoptical eye inspection, accurate quantification was realized by modified densitometric measurements: fluorescence mode, excitation wavelength 530 nm (D and W lamp) without optical filter, which offered satisfactory sensitivity (8.5–17.5 mg/kg) and accept- able linearity (R > 0.999 within 50–200 ng/zone). Moreover, the established method was validated with edible oil samples, against EU Directive 2006/52/EC. Apart from that, the unambiguous confirmation of positive results was conveniently achieved by TLC-MS interface-mediated mass spectrometry. Featuring the merits of screening conception, the proposed method not only reached the goal of accurate quantification and conclusive identification of multi-phenolic antioxidants, but also excellently balanced the simplicity, detectability, and throughput of the screening workup. Therefore, it might be an attractive alternative to conventional methods. . . . Keywords HPTLC DPPH Phenolic antioxidant Quantitative screening Introduction change, a large variety of antioxidants had http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Food Analytical Methods Springer Journals

Screening of Phenolic Antioxidants in Edible Oils by HPTLC-DPPH Assay and MS Confirmation

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Publisher
Springer US
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Chemistry; Food Science; Chemistry/Food Science, general; Microbiology; Analytical Chemistry
ISSN
1936-9751
eISSN
1936-976X
D.O.I.
10.1007/s12161-018-1295-x
Publisher site
See Article on Publisher Site

Abstract

The cytotoxicity of phenolic antioxidants had attracted marked attention, posing serious challenges to food safety. This paper presented a screening method for two major phenolic antioxidants (butylated hydroxytoluene and tert-butylhydroquinone) added in edible oils. To specifically visualize the targeted compounds after developing with toluene/ethyl acetate/methanol 8:1:1 (v/v/v) to 70 mm solvent front, the plate was subjected to a standardized 1,1-diphenyl-2-picrylhydrazyl assay. In addition to synoptical eye inspection, accurate quantification was realized by modified densitometric measurements: fluorescence mode, excitation wavelength 530 nm (D and W lamp) without optical filter, which offered satisfactory sensitivity (8.5–17.5 mg/kg) and accept- able linearity (R > 0.999 within 50–200 ng/zone). Moreover, the established method was validated with edible oil samples, against EU Directive 2006/52/EC. Apart from that, the unambiguous confirmation of positive results was conveniently achieved by TLC-MS interface-mediated mass spectrometry. Featuring the merits of screening conception, the proposed method not only reached the goal of accurate quantification and conclusive identification of multi-phenolic antioxidants, but also excellently balanced the simplicity, detectability, and throughput of the screening workup. Therefore, it might be an attractive alternative to conventional methods. . . . Keywords HPTLC DPPH Phenolic antioxidant Quantitative screening Introduction change, a large variety of antioxidants had

Journal

Food Analytical MethodsSpringer Journals

Published: May 31, 2018

References

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