Screening of high-quality bread wheat dihaploid lines by the use of biochemical markers

Screening of high-quality bread wheat dihaploid lines by the use of biochemical markers This work was undertaken to evaluate the storage protein composition of 13 doubled haploid bread wheat (Triticum aestivum L.) lines in order to identify those carrying promising alleles. For this, 15–20 seeds per each doubled haploid line were used to determine alleles at the loci for high-molecular-weight glutenin subunits and gliadins. Acid polyacrylamide gel electrophoresis was applied to identify glianin alleles, whereas SDS electrophoresis was used in the case of high-molecular-weight glutenin subunits. The identification of doubled haploid lines (DHLs) with respect to their storage protein composition enabled the classification of the DHLs derived from the cross Acheloos × Vergina in four different classes. Furthermore, protein composition analysis revealed that DHLs derived from the F1 Penios × Kavkaz were identical. In addition, these lines were found to carry 1BL/1RS translocation, which is associated with high yield potential and resistance to biotic and abiotic stress conditions. Finally, it was observed that, in two cases, one rare biotype of the parental varieties was involved in the crosses. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Screening of high-quality bread wheat dihaploid lines by the use of biochemical markers

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Publisher
Nauka/Interperiodica
Copyright
Copyright © 2006 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences; Plant Physiology
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443706030162
Publisher site
See Article on Publisher Site

Abstract

This work was undertaken to evaluate the storage protein composition of 13 doubled haploid bread wheat (Triticum aestivum L.) lines in order to identify those carrying promising alleles. For this, 15–20 seeds per each doubled haploid line were used to determine alleles at the loci for high-molecular-weight glutenin subunits and gliadins. Acid polyacrylamide gel electrophoresis was applied to identify glianin alleles, whereas SDS electrophoresis was used in the case of high-molecular-weight glutenin subunits. The identification of doubled haploid lines (DHLs) with respect to their storage protein composition enabled the classification of the DHLs derived from the cross Acheloos × Vergina in four different classes. Furthermore, protein composition analysis revealed that DHLs derived from the F1 Penios × Kavkaz were identical. In addition, these lines were found to carry 1BL/1RS translocation, which is associated with high yield potential and resistance to biotic and abiotic stress conditions. Finally, it was observed that, in two cases, one rare biotype of the parental varieties was involved in the crosses.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: May 15, 2006

References

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