ISSN 1021-4437, Russian Journal of Plant Physiology, 2006, Vol. 53, No. 3, pp. 396–400. © MAIK “Nauka /Interperiodica” (Russia), 2006.
Published in Russian in Fiziologiya Rastenii, 2006, Vol. 53, No. 3, pp. 444–448.
Dihaploidy is a technique that could accelerate the
production of a new cultivar . In the case of bread
wheat, it is an effective tool in reducing the time needed
to develop homozygous genotypes . The exploita-
tion of germplasm with desirable genes ensures the
development of good new individual combinations.
These combinations, however, have to be marked by
high yield and good quality to meet the demands of
modern agriculture. Good quality could be safely eval-
uated by comparing seed storage proteins of the dou-
bled haploid lines (DHL) to their original cultivars [3–5].
Wheat seed storage proteins belong to two major
groups: gliadins and high-molecular-weight glutenins.
Gliadins comprise a highly polymorphic group of alco-
hol-soluble endosperm proteins . The respective
loci, which are encoding gliadin production, are
A1, Gli-B1, Gli-D1, Gli-A3, Gli-A2, Gli-B2
. These loci are located on the following chromo-
some arms: 1AS, 1BS, 1DS, 1AS, 6AS, 6BS, and 6DS,
respectively. The loci encoding the production of high-
molecular-weight glutenin subunits are
, and they are located on 1AL, 1BL, and
1DL, respectively . Certain alleles have been found
to be associated with desirable traits. Allele
reveals the presence of 1BL/1RS translo-
cation. The last is associated with high yield potential
and resistance to biotic and abiotic stress conditions
[10, 19]. However, this translocation is responsible for
quality deterioration of the cultivars carrying it .
at gene locus
is associated with good
quality . In gene locus
also associated with good quality, whereas, in locus
are associated with better qual-
ity than allele
The evaluation of seed storage proteins can be per-
formed by various biochemical methods. These meth-
ods allow a more reliable study of the material, since
they are based on the genotype, and, therefore, they are
less inﬂuenced by the environment . Seed storage
protein electrophoresis (SSPE) and veriﬁcation of
existing alleles in corresponding loci facilitates the dis-
crimination and registration of the new germplasm [4,
5, 9]. Furthermore, SSPE could be used for the breeder
rights protection, homogeneity tests, and in evolution-
ary studies . For all the above reasons, high-molecu-
lar-weight (HMW) glutenin subunits are, respectively,
Screening of High-Quality Bread Wheat Dihaploid Lines
by the Use of Biochemical Markers
, I. N. Xynias
, I. Sozinov
, G. Lisova
, I. A. Zamani
, E. Gouli-Vavdinoydi
and D. G. Roupakias
Institute Plant Protection, Vasilkovska 33, Kiev, 03022 Ukraine
NAGREF–Cereal Institute of Thessaloniki, P.O. Box 411, 57001 Thermi, Thessaloniki, Greece;
Prefecture of Grevena, Division for Agricultural Development, Grevena, Greece
Laboratory of Genetics and Plant Breeding, School of Agronomy, Aristotle University of Thessaloniki, Greece
Received September 14, 2005
—This work was undertaken to evaluate the storage protein composition of 13 doubled haploid bread
L.) lines in order to identify those carrying promising alleles. For this, 15–20 seeds per
each doubled haploid line were used to determine alleles at the loci for high-molecular-weight glutenin subunits
and gliadins. Acid polyacrylamide gel electrophoresis was applied to identify glianin alleles, whereas SDS elec-
trophoresis was used in the case of high-molecular-weight glutenin subunits. The identiﬁcation of doubled haploid
lines (DHLs) with respect to their storage protein composition enabled the classiﬁcation of the DHLs derived from
the cross Acheloos
Vergina in four different classes. Furthermore, protein composition analysis revealed that
DHLs derived from the
Kavkaz were identical. In addition, these lines were found to carry 1BL/1RS
translocation, which is associated with high yield potential and resistance to biotic and abiotic stress conditions.
Finally, it was observed that, in two cases, one rare biotype of the parental varieties was involved in the crosses.
Key words: Triticum aestivum - doubled haploids - identiﬁcation - electrophoresis - glutenins - gliadins
: DHL—doubled haploid lines.
The text was submitted by the authors in English.