ISSN 1062-3604, Russian Journal of Developmental Biology, 2009, Vol. 40, No. 6, p. 395. © Pleiades Publishing, Inc., 2009.
Original Russian Text © V.Yu. Polyakov, 2009, published in Ontogenez, 2009, Vol. 40, No. 6, p. 480.
The scientiﬁc and practical conference on the modern
biological methods for reproductive medicine was held in
the Kol’tsov Institute of Developmental Biology, Russian
Academy of Sciences, on February 25th, 2009.
The goals of this conference were to expand the modern
concepts of the pathogenesis of some diseases affecting the
reproductive system, assist in introduction of the state-of-
the-art results of molecular biological and cellular studies
to the diagnostics of fertility abnormalities, and increase
the efﬁciency of infertility therapy and reproductive tech-
The presentation by L.F. Kurilo (Medical Genetic
Research Center of the Russian Academy of Medical Sci-
ences) considered the genetic mechanisms underlying
morphogenetic and functional pathologies of the reproduc-
tive system and the opportunities offered for diagnostics
and prevention of various pathologies.
E.E. Bragina (Belozersky Institute of Physicochemical
Biology, Moscow State University) presented the results of
ultrastructural studies of human spermatozoids. Quantita-
tive ultrastructural analysis of the chromatin in spermato-
zoids demonstrates that a 28% rate of spermatozoids with
abnormal chromatin condensation is critical for the devel-
opment of an embryo. Bragina also showed the data on the
intragametic herpes infection of human spermatozoids and
that an abnormality in the centriole structure could cause
idiopathic impairment of embryo development.
The topic of presentation by A.A. Kushch (Ivanovsky
Institute of Virology, Russian Academy of Medical Sci-
ences) was the study of the rate of detection of the herpes
simplex virus (HSV) in the sperm ejaculate of men with
impaired fertility and the evidence for a negative HSV
effect on sperm quality. HSV is signiﬁcantly more fre-
quently detected in both the whole ejaculate and the frac-
tion of actively moving spermatozoids of the man cohort
with primary infertility as compared with practically
healthy individuals. Infective HSV can be released in the
case of an asymptomatic herpes virus infection.
The lecture of a group of authors (A.Yu. Kulibin,
T.L. Marshak, and S.T. Zakhidov; Kol’tsov Institute of
Developmental Biology, Russian Academy of Sciences,
and Moscow State University) dwelled on the modern
concepts in the biology of spermatogonial stem cells
(SSC) and Sertoli cells (SSC niche) and illustrated suc-
cessful development of the methods for SSC isolation, cul-
tivation, and transplantation, offering great opportunities
for practical medicine, construction of transgenic animals,
preservation of rare and endangered species, and in vitro
modeling of spermatogenesis.
S.A. Yakovenko (Physical Faculty, Moscow State Uni-
versity) presented a developed original microscopic device
providing for resolving low-contrast vacuoles with a size of
m in the spermatozoid head. Use of spermato-
zoids without vacuoles considerably increases the rate of
successful pregnancies when using one of the reproductive
technologies---intracytoplasmic injection of spermatozo-
ids into the ovum.
After hearing and discussing the presentations, the con-
ference participants developed recommendations underlin-
(1) It is necessary to develop the standards (protocols)
for combined examination of the patients with reproductive
pathologies using state-of-the-art genetic, cytological,
ultrastructural, and molecular biological techniques;
(2) It is purposeful to use ultrastructural, virological,
and cytogenetic analyses of male gametes in the case of
miscarriages and unsuccessful application of reproductive
(3) It is purposeful to discuss the topical problems con-
nected with the deﬁciency in medical geneticists and the
staff of some other specialties required for complex diag-
nostic and research procedures (cytogenetics), because
biologists cannot attend certiﬁcation courses on a regular
(4) The scientiﬁc developments in the ﬁeld of diagnos-
tics, therapy, and prevention of abnormalities in the repro-
ductive health under the national project of demographic
development in Russia are a priority direction.
It was proposed to organize on a regular basis the con-
ferences that would inform reproductologists, gynecolo-
gists, andrologists, and laboratory staff working in practical
medicine about the relevant modern biological methods.
Scientific and Practical Conference “Modern Biological Methods
for Reproductive Medicine”
(February 25, 2009, Moscow, Russia)