A scale-up approach was investigated for maximum production of cholesterol oxidase (CO) enzyme, due to its high demand in agricultural and pharmaceutical industries. The CO production from Streptomyces rimosus in submerged fermentation conditions at shake flask level was observed in yeast malt glucose (YMG) medium as 1.82 U/mL. Maximum specific growth rate (μmax) and generation time (g) in log phase of microbial growth were found as 0.107 h−1 and 6.46 h respectively. During the CO production in 3-L bioreactor, the optimum parameters were observed as agitation—200 rpm, aeration—1.5 vvm, pH—7.0 and temperature 30 °C, with maximum production (8.66 U/mL, yield 0.916 U/mg cells), which showed 4.75 fold increase as compared to shake-flask production (1.82 U/mL, yield 0.243 U/mg). The volumetric oxygen mass transfer rate (KLa) was found to be in the range of 16 h−1 by dynamic gassing out method under optimized aeration and agitation parameters. The production of CO from S. rimosus in bioreactor is not reported earlier.
Environmental Sustainability – Springer Journals
Published: Jun 4, 2018
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera