Scale up and optimization of cholesterol oxidase production from Streptomyces rimosus MTCC 10792 in a 3-L bioreactor

Scale up and optimization of cholesterol oxidase production from Streptomyces rimosus MTCC 10792... A scale-up approach was investigated for maximum production of cholesterol oxidase (CO) enzyme, due to its high demand in agricultural and pharmaceutical industries. The CO production from Streptomyces rimosus in submerged fermentation conditions at shake flask level was observed in yeast malt glucose (YMG) medium as 1.82 U/mL. Maximum specific growth rate (μmax) and generation time (g) in log phase of microbial growth were found as 0.107 h−1 and 6.46 h respectively. During the CO production in 3-L bioreactor, the optimum parameters were observed as agitation—200 rpm, aeration—1.5 vvm, pH—7.0 and temperature 30 °C, with maximum production (8.66 U/mL, yield 0.916 U/mg cells), which showed 4.75 fold increase as compared to shake-flask production (1.82 U/mL, yield 0.243 U/mg). The volumetric oxygen mass transfer rate (KLa) was found to be in the range of 16 h−1 by dynamic gassing out method under optimized aeration and agitation parameters. The production of CO from S. rimosus in bioreactor is not reported earlier. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Environmental Sustainability Springer Journals

Scale up and optimization of cholesterol oxidase production from Streptomyces rimosus MTCC 10792 in a 3-L bioreactor

Loading next page...
 
/lp/springer_journal/scale-up-and-optimization-of-cholesterol-oxidase-production-from-rff9rqjUMb
Publisher
Springer Singapore
Copyright
Copyright © 2018 by Society for Environmental Sustainability
Subject
Environment; Sustainable Development; Environment, general; Environmental Management; Climate Change; Natural Resources
eISSN
2523-8922
D.O.I.
10.1007/s42398-018-0008-0
Publisher site
See Article on Publisher Site

Abstract

A scale-up approach was investigated for maximum production of cholesterol oxidase (CO) enzyme, due to its high demand in agricultural and pharmaceutical industries. The CO production from Streptomyces rimosus in submerged fermentation conditions at shake flask level was observed in yeast malt glucose (YMG) medium as 1.82 U/mL. Maximum specific growth rate (μmax) and generation time (g) in log phase of microbial growth were found as 0.107 h−1 and 6.46 h respectively. During the CO production in 3-L bioreactor, the optimum parameters were observed as agitation—200 rpm, aeration—1.5 vvm, pH—7.0 and temperature 30 °C, with maximum production (8.66 U/mL, yield 0.916 U/mg cells), which showed 4.75 fold increase as compared to shake-flask production (1.82 U/mL, yield 0.243 U/mg). The volumetric oxygen mass transfer rate (KLa) was found to be in the range of 16 h−1 by dynamic gassing out method under optimized aeration and agitation parameters. The production of CO from S. rimosus in bioreactor is not reported earlier.

Journal

Environmental SustainabilitySpringer Journals

Published: Jun 4, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off