ISSN 10227954, Russian Journal of Genetics, 2010, Vol. 46, No. 8, pp. 917–923. © Pleiades Publishing, Inc., 2010.
Original Russian Text © J.L. Kuzmina, V.V. Panov, N.E. Vorobyeva, N.V. Soshnikova, M.R. Kopantseva, J.V. Nikolenko, E.N. Nabirochkina, S.G. Georgieva, Yu.V. Shidlovskii,
2010, published in Genetika, 2010, Vol. 46, No. 8, pp. 1033–1040.
Transcription activation by RNA polymerase II in
higher eukaryotes requires the participation of numer
ous factors, including general transcription factors
(GTFs), genespecific activators, and coactivators.
Coactivators serve two main functions: chromatin
remodeling and recruitment of GTFs to the promoter
[1–3]. Many proteins and protein complexes are
known to act as coactivators, and their number is still
increasing. A novel coactivator SAYP involved in tran
scription of many genes in
was described by
us recently [4, 5].
MATERIALS AND METHODS
was carried out accord
ing to . The probes were DNA fragments corre
sponding to exon 2 of the
gene and to a fullsize
In situ hybridization
was carried out as described in
. The probe was a DNA fragment complementary
to the coding strand of exons 5–12 of the
fragment complementary to the noncoding strand of
the same region of the
gene was used in the con
. Antibodies against cyclin B
(Developmental Studies Hybridoma Bank), SAYP
, and PHF10 were used. The antibodies against the
PHF10 protein were raised in rabbits immunized with
a recombinant protein expressed in
. DNA was
stained with DAPI.
Human Normal & Tumor
Tissue Lysate Array kit (ThermoScientific) was used to
study the distribution of the PHF10 protein in differ
ent human tissues.
Multistep chromatography and purification of the
were performed as described in .
RESULTS AND DISCUSSION
SAYP Is a Factor with Regulated Expression
gene encoding SAYP was originally iden
tified as a gene required for the enhancer–promoter
interaction in the
gene . Therefore, the prod
uct of the
gene was called Supporter of Activa
tion of Yellow Protein (SAYP) . The
located on the X chromosome, it is about 10.5 kb in
length and has two promoters and two adenylation
sites. Some of its transcripts have a long 3'untrans
lated region (3'UTR) carrying AREs that presumably
affect mRNA stability . The 3'UTR of the
gene also contains the
gene oriented oppo
. Some data indicate that
are inversely correlated in their expression (Fig. 1a),
which suggests the RNA interference mechanism of
regulation in this case, as shown, for example, for the
SAYP is a protein consisting of 2008 amino acids.
Its homologs are found in all metazoans, and all have
a conserved core consisting of the SAY domain and
two zinc finger domains of the PHD type (Fig. 1b).
However, all homologs differ in the sequences outside
the conserved core. In
, this protein has two
positively charged regions and an AThook motif
needed for DNA binding.
SAYP Is a Novel Regulator of Metazoan Development
J. L. Kuzmina
, V. V. Panov
, N. E. Vorobyeva
, N. V. Soshnikova
, M. R. Kopantseva
J. V. Nikolenko
, E. N. Nabirochkina
, S. G. Georgieva
, and Yu. V. Shidlovskii
Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119991 Russia;
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 117984 Russia
University of Oslo, Centre for Medical Studies, Moscow, 119334 Russia
Received February 10, 2010
—SAYP is a dualfunction transcriptional coactivator of RNA polymerase II. It is a metazoanspe
cific factor involved in different signaling pathways that control normal development. In
, SAYP is
present in the organism from the early stages of development and participates in cell cycle synchronization at
the blastoderm stage. SAYP is abundant in many embryonic cells and in imaginal discs of larvae and is crucial
for oogenesis in adults. At the molecular level, SAYP serves as a basis for assembling the BTFly nuclear super
complex consising of the Brahma and TFIID coactivators. We suppose that BTFly and other similar nuclear
supercomplexes play an important role in ontogenesis.