ISSN 10214437, Russian Journal of Plant Physiology, 2010, Vol. 57, No. 6, pp. 784–791. © Pleiades Publishing, Ltd., 2010.
The halophytic plant,
Poaceae family is a stoloniferous perennial grass that
type of photosynthesis. It is distributed in the
regions with intermediate salinity and semidesert cli
mate on Iranian plateau and is one of the animal for
age plants . Vegetative propagation is one of the
to produce identical plant
lines, which are needed for investigation of molecular
and physiological aspects of adaptation to harsh envi
ronmental conditions. The patterns of ion secretion by
as a powerful strategy to cope with salt
stress were reported .
is very important
plant because it can tolerate high salt and drought
stress levels; it belongs to Poaceae, which is an impor
tant family of crops, including wheat and rice. Colmer
This text was submitted by the authors in English.
et al.  have reported that it is possible to use wild rel
atives to improve wheat salt tolerance. Wei et al. 
have reported the transfer of salt tolerance to wheat
, which is its wild and remote relative.
To investigate the salt tolerance transfer to hybrid
clones, proline content and the Na
analyzed, and these two parameters were increased in
hybrid clones under salt stress .
NaCl inhibits plant growth by osmotic or water
deficit effect and saltspecific or ionexcess effect .
Several roles for proline in plants are osmotic adjust
ment, stabilizing subcellular structures, scavenging
free radicals, and buffering the cell redox potential
under stress conditions . Proline can also induce
expression of salt stressresponsive genes, which have
prolineresponsive elements in their promoters .
Earlier, it has been shown that proline content in
was dramatically increased under
drought stress .
The effect of salt stress on plant growth depends on
the plant species. In
, fresh and dry
Salt Stress Responses of a Halophytic Grass
and Subsequent Recovery
a, b, c, d
, Nasrin Motamed
, Ferdous Rastgar Jazii
, Khadija Razavi
, and Setsuko Komatsu
School of Biology, College of Science, University of Tehran, Tehran 141556455, Iran;
fax: +982166405141; email: email@example.com
National Research Center for Genetic Engineering and Biotechnology, Tehran 141556343, Iran
National Institute of Crop Science, Tsukuba 3058518, Japan
Payame Noor University, Tehran 193954697, Iran
Received February 25, 2009
—To investigate the salt tolerance mechanisms,
as a halophytic plant was used.
Plants were treated with 0, 150, 450, 600, and 750 mM NaCl and harvested at 0, 4, 8, and 10 days after treat
ment and 1 day and 1 week after recovery. Optimal growth, measured as fresh and dry weights, occurred at
150 mM NaCl, but it was suppressed by 450, 600, and 750 mM NaCl. Recovery significantly increased fresh
and dry weights only in 750 mM NaCltreated plants. Water content was decreased after NaCl treatment and
increased after recovery. Na
and proline contents and activity of superoxide dismutase (SOD) were
increased after NaCl treatment and decreased after recovery in all treated plants. In contrast, K
ascorbate peroxidase activity decreased after NaCl treatment and increased after recovery in all treated plants.
Catalase (CAT) was activated only in 750 mM NaCltreated plants. Total content of soluble protein was
slightly changed after NaCl treatment. It was concluded that proline accumulation for osmotic adjustment,
SOD activation for scavenging, and CAT activation at the higher level of salt stress to detoxify produced
strategies under salt stress.
salt tolerance was not based on the
restriction of Na
Aeluropus lagopoides, antioxidant enzymes, growth, halophyte, ion content, proline, recovery, salt
APX—ascorbate peroxidase; CAT—catalase;