Salicylic acid induces the change in the adventitious root of Glycyrrhiza uralensis Fisch.: bioactive compounds and antioxidant enzymes

Salicylic acid induces the change in the adventitious root of Glycyrrhiza uralensis Fisch.:... In this study, adventitious roots of Glycyrrhiza uralensis were cultured in a 5-L balloon-type bubble bioreactor with different medium salt strength and different concentrations of sucrose. The results of the culture showed that the best culture conditions were 4 % sucrose concentration and 1 MS medium for the accumulation of secondary metabolites. Salicylic acid has previously been used as an elicitor in tissue cultures to enhance production of secondary metabolites. Addition of 1 mg L−1 salicylic acid significantly enhanced the contents of glycyrrhizic acid (0.31 mg g−1), glycyrrhetinic acid (0.14 mg g−1) and polysaccharide (159.29 mg g−1) in the G. uralensis adventitious roots and the contents were 2.58-fold, 1.27-fold, and 2.07-fold over the control group. Furthermore, the greatest content of total flavonoid (9.40 mg g−1) was observed with 2 mg L−1 salicylic acid added in the culture, which was 2.68-fold higher than the control. The ESI-MSn analysis was also performed, showing that two new kinds of flavonoids, including (3R)-vestitol and glycyrol, were only identified in the salicylic acid treatment. In addition, we also found that salicylic acid significantly increased superoxide dismutases, catalases, and peroxidase activity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Research on Chemical Intermediates Springer Journals

Salicylic acid induces the change in the adventitious root of Glycyrrhiza uralensis Fisch.: bioactive compounds and antioxidant enzymes

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Publisher
Springer Netherlands
Copyright
Copyright © 2015 by Springer Science+Business Media Dordrecht
Subject
Chemistry; Catalysis; Physical Chemistry; Inorganic Chemistry
ISSN
0922-6168
eISSN
1568-5675
D.O.I.
10.1007/s11164-015-2099-x
Publisher site
See Article on Publisher Site

Abstract

In this study, adventitious roots of Glycyrrhiza uralensis were cultured in a 5-L balloon-type bubble bioreactor with different medium salt strength and different concentrations of sucrose. The results of the culture showed that the best culture conditions were 4 % sucrose concentration and 1 MS medium for the accumulation of secondary metabolites. Salicylic acid has previously been used as an elicitor in tissue cultures to enhance production of secondary metabolites. Addition of 1 mg L−1 salicylic acid significantly enhanced the contents of glycyrrhizic acid (0.31 mg g−1), glycyrrhetinic acid (0.14 mg g−1) and polysaccharide (159.29 mg g−1) in the G. uralensis adventitious roots and the contents were 2.58-fold, 1.27-fold, and 2.07-fold over the control group. Furthermore, the greatest content of total flavonoid (9.40 mg g−1) was observed with 2 mg L−1 salicylic acid added in the culture, which was 2.68-fold higher than the control. The ESI-MSn analysis was also performed, showing that two new kinds of flavonoids, including (3R)-vestitol and glycyrol, were only identified in the salicylic acid treatment. In addition, we also found that salicylic acid significantly increased superoxide dismutases, catalases, and peroxidase activity.

Journal

Research on Chemical IntermediatesSpringer Journals

Published: May 24, 2015

References

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